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Caspase 7 d2q3l

Manufactured by Cell Signaling Technology
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Sourced in United States

Caspase-7 (D2Q3L) is a lab equipment product from Cell Signaling Technology. It is a recombinant rabbit monoclonal antibody that recognizes Caspase-7, a member of the cysteine-aspartic acid protease (caspase) family. Caspases play a central role in the execution of the cell apoptosis (programmed cell death) program.

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Lab products found in correlation

Caspase 3, by Cell Signaling Technology (2 mentions) β actin 1 19, by Santa Cruz Biotechnology (1 mentions) Caspase 9, by Cell Signaling Technology (1 mentions) Cleaved caspase 7, by Cell Signaling Technology (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Cleaved caspase 9, by Cell Signaling Technology (1 mentions) Ripa buffer, by Cell Signaling Technology (1 mentions) Active caspase 3, by Cell Signaling Technology (1 mentions) P fak y576 577, by Cell Signaling Technology (1 mentions) P paxillin y118, by Cell Signaling Technology (1 mentions) P130cas, by BD (1 mentions) Hsp90, by Cell Signaling Technology (1 mentions) Mmp 2, by Cell Signaling Technology (1 mentions) Mmp 9, by Cell Signaling Technology (1 mentions) Pakt s473, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Gdc 0941, by LC Laboratories (1 mentions) Saracatinib, by LC Laboratories (1 mentions) Antibody against β actin, by Merck Group (1 mentions)

3 protocols using caspase 7 d2q3l

1

Apoptosis Signaling Pathway Analysis

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HeLa and C33A cells were treated with the indicated drugs and harvested. Cell pellets were lysed in RIPA buffer (Cell Signaling Technology) containing 1mM PMSF. Equal quantities of proteins (50 µg) were separated by 10% precast polyacrylamide gels and transferred onto nitrocellulose membranes. Membranes were incubated with the following primary antibodies: Cleaved PARP (Asp214) (D64E10), PARP, Cleaved Caspase-3 (Asp175) (5A1E), Caspase-3 (D3R6Y), Cleaved Caspase-9 (Asp330) (E5Z7N), Caspase-9 (C9), Cleaved Caspase-7 (Asp198) (D6H1), Caspase-7 (D2Q3L) (Cell Signaling Technologies); and β-Actin (I-19) (Santa Cruz Biotechnology) and further analyzed following the manufacturer’s recommendations.
McDonnell A.M., Pyles H.M., Diaz-Cruz E.S, & Barton C.E. (2019). Enoxacin and Epigallocatechin Gallate (EGCG) Act Synergistically to Inhibit the Growth of Cervical Cancer Cells in Culture. Molecules, 24(8), 1580.
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2

Antibody and Chemical Reagents Protocol

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Antibodies against P-FAK (Y576/577) (#3281), P-Paxillin (Y118) (#2541), P-p130Cas (Y410) (#4011), PARP (#9542), Akt (#9272), Src (#2109), Active-Caspase-3 (#9664), P-Akt (S473) (#4051), Caspase 7 (D2Q3L) (#12827), VHL (#2738), MMP2 (#4022), MMP9 (#3852) and HSP90 (#4874) were from Cell Signaling Technologies (Danvers, MA). Antibodies against Paxillin (610052), FAK (610088) and p130Cas (610271) were from BD Biosciences (San Jose, CA). Antibody against P-Src (Y418) (LF-PA20465) was purchased from AbFrontier. Antibody against PCNA was from Abcam (Cambridge, UK). GAPDH (AM4300) was purchased from Life Technologies (Carlsbad, CA). Antibody against HA-12CA5 (11666606001) was from Roche. Dimethyl sulfoxide was from Sigma Aldrich (St Louis, MO). Saracatinib and GDC-0941 were obtained from LC Laboratories (Woburn, MA).
Roelants C., Giacosa S., Pillet C., Bussat R., Champelovier P., Bastien O., Guyon L., Arnoux V., Cochet C, & Filhol O. (2018). Combined inhibition of PI3K and Src kinases demonstrates synergistic therapeutic efficacy in clear-cell renal carcinoma. Oncotarget, 9(53), 30066-30078.
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3

Immunoblotting Procedure for Cell Signaling Proteins

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Antibodies against IκBα (#9242), phospho-SAPK/JNK (Thr183/Tyr185; #9251), SAPK/JNK (#9252), phospho-p38 MAPK (Thr180/Tyr182; #9211), p38 MAPK (D13E1) XP rabbit (#8690), PARP (46D11) rabbit (#9532), Caspase 3 (#9662), Caspase 7 (D2Q3L; #12827), LC3A/B (D344C) XP rabbit (#12741), anti-mouse IgG (#7076), and anti-rabbit IgG (#7074) were purchased from Cell Signaling Technology (Danvers, MA, USA). Dox (#D1515) and antibody against β-actin (#A2228) were obtained from Sigma-Aldrich. Antibody against MKP-1 (M-18) was purchased from Santa Cruz Biotechnology (#sc-1102, Dallas, TX, USA). MLN9708 (#A4007) was purchased from ApexBio (Houston, TX, USA) and was prepared according to the manufacturer’s recommendations.
Wang H., Yu Y., Jiang Z., Cao W.M., Wang Z., Dou J., Zhao Y., Cui Y, & Zhang H. (2016). Next-generation proteasome inhibitor MLN9708 sensitizes breast cancer cells to doxorubicin-induced apoptosis. Scientific Reports, 6, 26456.
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