The cDNA of male–sterile lines HL and LL was used as the template for the PCR amplification. The Primer–BLAST program was used to design primers specific for the coding sequence (CDS) regions of selected DEGs (Supplementary Table S4 ). The amplified products were purified using the SanPrep Column DNA Gel Extraction Kit (Sangon Biotech, Shanghai, China) and inserted into the pEASY–Blunt vector (TransGen Biotech) using the pEASY®–Blunt Cloning Kit (TransGen Biotech). The inserted fragments were amplified with PCR using the M13F/R primers and then sequenced (Sangon Biotech, Shanghai, China).
M13f r primers
Manufactured by Sangon
Sourced in China
M13F/R primers are short DNA sequences used in molecular biology applications to amplify and sequence DNA fragments. They serve as universal primers that can be used to amplify and sequence inserted DNA fragments in cloning vectors, such as M13 phage-derived plasmids.
Automatically generated - may contain errors
2 protocols using m13f r primers
1
Cloning and Sequencing of DEGs
2
Cloning and Sequencing of DEGs
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The cDNA of male–sterile lines HL and LL was used as the template for the PCR amplification. The Primer–BLAST program was used to design primers specific for the coding sequence (CDS) regions of selected DEGs (Supplementary Table S4 ). The amplified products were purified using the SanPrep Column DNA Gel Extraction Kit (Sangon Biotech, Shanghai, China) and inserted into the pEASY–Blunt vector (TransGen Biotech) using the pEASY®–Blunt Cloning Kit (TransGen Biotech). The inserted fragments were amplified with PCR using the M13F/R primers and then sequenced (Sangon Biotech, Shanghai, China).
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