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Digital video camera

Manufactured by Sony
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The Digital Video Camera is a high-performance camera designed for capturing and recording video. It features advanced image sensors, a robust built-in storage system, and intuitive controls for easy operation. The camera is capable of recording video in a variety of resolutions and formats, making it a versatile tool for various applications.

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Lab products found in correlation

Celltrace cfse cell proliferation kit, by Thermo Fisher Scientific (2 mentions) Ckx41, by Olympus (1 mentions) Ckx41 fluorescence microscope, by Olympus (1 mentions)

9 protocols using digital video camera

1

CTC-chip cell capture and proliferation

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Cells were labeled using the CellTrace™ CFSE Cell Proliferation kit (Life Technologies) as per the manufacturer's protocol, and then about 1,500 PC3 and LNCaP cells were suspended in 2 mL of PBS containing 5% BSA or in 2 mL of blood sampled from a healthy volunteer. A cell suspension sample of 1 mL (500 cells/mL) was applied to the CTC-chip system.
Each sample was sent into the chip using a syringe pump at a constant flow rate (1.5 mL/h when suspended in PBS or 1.0 mL/h when suspended in blood). Each sample tube was shaken to ensure that the cell suspension was homogeneous. Images and videos of the cells in the chip were monitored and recorded with a fluorescence microscope (CkX41; Olympus) and a digital video camera (Sony Biotechnology, Inc., Tokyo, Japan). Each experiment, sample preparation, and flow test were performed five times.
Obayashi K., Akatsuka J., Endo Y., Takeda H., Hayashi T., Toyama Y., Suzuki Y., Hamasaki T., Kimura G., Ohnaga T, & Kondo Y. (2019). Initial detection of circulating tumor cells from metastatic prostate cancer patients with a novel small device. Prostate International, 7(4), 131-138.
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2

Nest Building Ability Assessment in Mice

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Nest building test was performed as previously described [61 (link)]. To test nest building ability of mice, one square piece of nesting material made of cotton fiber (5 × 5 cm) was introduced to a cage in which mouse was individually housed. Pictures of the nests were taken 24 h later with a Sony digital video camera. The quality of the nest was assessed using the following score criteria: 1_nestlet not noticeable touched, 2_nestlet partially torn up, 3_ mostly shredded but not identifiable nest site, 4_an identifiable but flat nest, 5_ a well-defined nest with walls.
Dong J., Chen W., Liu N., Chang S., Zhu W, & Kang J. (2022). NRG1 knockdown rescues PV interneuron GABAergic maturation deficits and schizophrenia behaviors in fetal growth restriction mice. Cell Death Discovery, 8, 476.
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3

Sociability and Social Novelty Tests

Cited 1 time
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Sociability and social novelty tests were performed as previously described [60 (link)]. Sociability and social novelty were analyzed in an arena (60 × 60 cm) with two equidistant clear Plexiglas cylinders (each 7 cm in diameter, 12 cm tall). The cylinders had multiple holes (1 cm in diameter) to allow the auditory, visual and olfactory interaction between the stimulus mouse placed inside and the test mouse outside of the cylinder. Between trials, the apparatus and cylinders were cleaned with 75% ethanol and dried before testing the next mouse. Time sniffing the social and non-social cylinders was operationally defined as the time during which the test mouse made direct nose-to-cylinder contact with the social and non-social cylinders, respectively. This was captured and recorded with Sony digital video camera mounted above the chamber. The paradigm consisted in a three-stage procedure: During habituation phase, the testing mouse was allowed to initially explore the apparatus with the cylinders for 10 min. In social approaching phase, an unfamiliar sex-matched mouse was placed in one of the cylinder, maintaining the other empty. Finally, in social novelty test, another unfamiliar mouse was introduced to the other empty cylinder. In the preference for social novelty test, preference score was calculated by subtracting the time spent sniffing the two stranger mice.
Dong J., Chen W., Liu N., Chang S., Zhu W, & Kang J. (2022). NRG1 knockdown rescues PV interneuron GABAergic maturation deficits and schizophrenia behaviors in fetal growth restriction mice. Cell Death Discovery, 8, 476.
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4

Basso-Beattie-Bresnahan Locomotor Assessment

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Locomotor function was evaluated using the Basso-Beattie-Bresnahan (BBB) locomotor rating scale [30 (link)]. The open-field environment was a molded plastic wading pool with a nonslippery surface (100 cm in diameter and 21 cm in height) [30 (link)]. The BBB scores were assessed weekly after the surgery. Seven subjects were randomly selected for testing, which lasted for approximately 4 min and was recorded using a digital video camera (Sony). The hindlimb movement scores were assessed by two independent observers blinded to the group identities.
Yuan T., Liu Q., Kang J., Gao H, & Gui S. (2019). High-Dose Neural Stem/Progenitor Cell Transplantation Increases Engraftment and Neuronal Distribution and Promotes Functional Recovery in Rats after Acutely Severe Spinal Cord Injury. Stem Cells International, 2019, 9807978.
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5

Evaluation of Tumor Cell Capture Efficiency

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Sample preparation and flow test to evaluate cell‐capture efficiency were carried out as described previously.13 In brief, tumor cells were labeled with CellTrace CFSE Cell Proliferation Kit (Life Technologies, Carlsbad, CA, USA), and were suspended either in PBS containing 5% BSA or in the blood sampled from a healthy volunteer at the concentration of 100 and 500 cells/mL. A cell suspension sample of 1 mL, which contains 100 or 500 tumor cells, was applied to the CTC‐chip.
Images and movies of cells in the CTC‐chip were monitored and recorded with a CKX41 fluorescence microscope (Olympus, Tokyo, Japan) and a digital video camera (Sony). The actual number of cells that were sent into the chip (N‐total) was determined by counting the number of cells that passed through the inlet of the CTC‐chip, and the number of captured cells (N‐captured) was also determined by counting CFSE‐labelled cells remained on the CTC‐chip. The cell capture efficiency was represented as N‐captured/N‐total.12, 13, 14 Experiments were carried out in triplicate.
Yoneda K., Kuwata T., Chikaishi Y., Mori M., Kanayama M., Takenaka M., Oka S., Hirai A., Imanishi N., Kuroda K., Ichiki Y., Ohnaga T, & Tanaka F. (2019). Detection of circulating tumor cells with a novel microfluidic system in malignant pleural mesothelioma. Cancer Science, 110(2), 726-733.
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6

Open Field Test for Mouse Behavior

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The open field (OFT) was designed following the procedure described by Heimrich et al. [21 (link)]. Each mouse was placed in the middle of an open box (50 × 50 × 50 cm) and the area was divided into 9 squares (16.67 cm × 16.67 cm per square) and painted with white line. During the 10 min test, mouse activity was recorded by a digital video camera (SONY, Japan) and analyzed by Top Scan software (Super Maze V2.0, XinRuan Information Technology Co. Ltd., Quanzhou, China). Finally, these parameters including the numbers of those crossing the central grid, times of climbing and standing, total numbers of crossing grids were analyzed.
Lin L., Zhang J., Dai X., Xiao N., Ye Q, & Chen X. (2022). A Moderate Duration of Stress Promotes Behavioral Adaptation and Spatial Memory in Young C57BL/6J Mice. Brain Sciences, 12(8), 1081.
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7

Light/Dark Transition Test in Mice

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On day 16, the LDTT was conducted (Figure 1B), as formerly defined (Li et al., 2006 (link); Martinho et al., 2020 (link); Silvestri et al., 2020 (link); Martinho et al., 2021 (link)). The apparatus consisted of a wooden box without a lid comprising a light (50 × 30) and a dark compartment (50 × 20) with 25 cm high walls. The different areas were joined by a square entrance (10 × 10 cm), which remained open during the entire duration of the test. The test started by placing each animal into the light area facing the opposite wall to the entrance. A Sony digital video camera (Sony Corporation, Japan) was positioned above the apparatus and used to record the mice’s behavior, namely the time spent in the light area, the number of passages to the light area, and the total number of passages between dark and light areas. The test lasted for a total of 5 min, and results were obtained from manual and blind analyzes of the resulting videos.
Martinho R., Seixas R., Azevedo M., Oliveira A., Serrão P, & Moreira-Rodrigues M. (2022). Sotalol Treatment may Interfere With Retrieval, Expression, and/or Reconsolidation Processes Thus Disrupting Traumatic Memories in a Post-Traumatic Stress Disorder Mice Model. Frontiers in Pharmacology, 12, 809271.
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8

Evaluating Kinematics and Performance in Soccer Kicks

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Participants completed three sessions within this study. The first session consisted of the pre-test and collection of basic demographic information including: age, footedness (self-report), and a kicking experience questionnaire. Participants then completed a 20 kick warm-up period, where they were allowed to kick the ball to the target using either foot. The pre-test consisted of the completion of 26 soccer (instep) kicks with both the dominant and non-dominant foot (13 each side; See Supplementary Video File). The order of kicks was randomized across participants in order to minimize learning effects. During the pre-test, participants were filmed from behind and slightly to the side using a SONY digital video camera at 25 fps. This video footage was then used to create practice training DVDs and showed kinematic data (markers located on ankle, knee, hip, wrist, elbow, and shoulder), ball path, and scoring result to the participant.
Steel K.A, & Ellem E. (2016). Is Mixed Practice More Effective than Physical Practice Alone for the Acquisition of Non-dominant Side Kicking Performance?. Frontiers in Psychology, 7, 1665.
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9

Facial Paralysis Assessment Protocol

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Institutional review board approval was obtained by the MEEI Human Study Committee and written informed consent was obtained for all participants. We administered the SSA to a group of 100 healthy individuals (control group) and to a group of 30 patients with facial paralysis, from July 1, 2014, to December 31, 2014. The SSA was displayed on an iPad (Apple Inc) and a Sony Digital video camera (Sony Corporation) was used to videotape participants while they were watching the SSA. Both the iPad and video camera were fixed on a tripod. Participants were asked to watch the video while sitting on a chair at a distance of 100 cm from the tripod. To facilitate facial measurements and evaluation, a headrest was used to reduce head movements, and the midline position of the participant's face was aligned with a pointer (Figure 1).
Iacolucci C.M., Banks C., Jowett N., Kozin E.D., Bhama P.K., Barbara M, & Hadlock T.A. (2015). Development and validation of a spontaneous smile assay. JAMA facial plastic surgery, 17(3).
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