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Xbridge peptide beh c18 prep 130 5 μm column 10 150 mm

Manufactured by Waters Corporation
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The XBridge® Peptide BEH C18 Prep 130 Å 5 μm column (10 × 150 mm) is a preparative-scale reversed-phase liquid chromatography column designed for the purification of peptides. The column features a particle size of 5 μm and a pore size of 130 Å, which is suitable for the separation and isolation of a wide range of peptide molecules.

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XBridge BEH 130 Prep C18 Column XBridge Peptide BEH C18 column XBridge™ BEH 130 C18 XBridge Peptide BEH C18 XBridge® Prep BEH C18 XBridge peptide BEH column XBridge BEH C18 column XBridge Prep C18 column Peptide BEH C18 column Xbridge C18 5 μm

2 protocols using xbridge peptide beh c18 prep 130 5 μm column 10 150 mm

1

HPLC Purification of Crude Peptides

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Crude samples (25 mg/mL; 40 μL injection) were separated using a XBridge® Peptide BEH C18 Prep 130 Å 5 μm column (10 × 150 mm) (Waters) with a binary eluent system comprising MeOH and H2O (with 0.1% v/v formic acid) as mobile phase. Operating pressures were around 2000 PSI. Isolated pure peptides were concentrated in vacuo to remove organic volatiles and the aqueous solutions were then flash frozen (liquid N2) and lyophilised.
von Mentzer B., Russo A.F., Zhang Z., Kuburas A., Killoran P.M., D’Aloisio V., Nizic L., Capel V., Kendall D.A., Coxon C.R, & Hutcheon G.A. (2020). A CGRP receptor antagonist peptide formulated for nasal administration to treat migraine. The Journal of Pharmacy and Pharmacology, 72(10), 1352-1360.
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2

Peptide Isolation and Purification

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Crude samples (25 mg/ml; 40 μl injection) were separated using a XBridge Peptide BEH C18 Prep 130 Å 5 μm column (10 × 150 mm) (Waters) with a binary eluent system comprising MeOH and H2O (with 0.1% v/v formic acid) as mobile phase. Operating pressures were around 2000 PSI. Isolated pure peptides were concentrated in vacuo to remove organic volatiles, and the aqueous solutions were then flash-frozen (liquid N2) and lyophilised.
von Mentzer B., Russo A.F., Zhang Z., Kuburas A., Killoran P.M., D’Aloisio V., Nizic L., Capel V., Kendall D.A., Coxon C.R, & Hutcheon G.A. (2020). A CGRP receptor antagonist peptide formulated for nasal administration to treat migraine. The Journal of Pharmacy and Pharmacology, 72(10), 1352-1360.
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