Log In Sign up
The largest database of trusted experimental protocols

Stearoyl coa desaturase 1

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

Stearoyl-CoA desaturase 1 is an enzyme that catalyzes the rate-limiting step in the biosynthesis of monounsaturated fatty acids. It introduces a double bond into stearoyl-CoA, converting it to oleoyl-CoA.

Automatically generated - may contain errors

Lab products found in correlation

Fatty acid synthase, by Cell Signaling Technology (2 mentions) Bromodeoxyuridine (brdu), by Cell Signaling Technology (1 mentions) Acetyl coa carboxylase, by Cell Signaling Technology (1 mentions) Phospho s6 ribosomal protein s235 236, by Cell Signaling Technology (1 mentions) Phospho erk t202 y204, by Cell Signaling Technology (1 mentions) Total s6 ribosomal protein, by Cell Signaling Technology (1 mentions) Bolt bis tris plus polyacrylamide gels, by Thermo Fisher Scientific (1 mentions) Total s6 kinase, by Cell Signaling Technology (1 mentions) Anti cpt1a antibody, by Abcam (1 mentions) Phospho s6 kinase, by Cell Signaling Technology (1 mentions) Tuberin tsc2, by Cell Signaling Technology (1 mentions) Phospho rsk s380, by Cell Signaling Technology (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) N cadherin, by Santa Cruz Biotechnology (1 mentions) Phospho smad2 3, by Cell Signaling Technology (1 mentions) Total acetyl coa carboxylase, by Cell Signaling Technology (1 mentions) Total oxphos human wb antibody cocktail, by Abcam (1 mentions) Phospho acetyl coa carboxylase, by Cell Signaling Technology (1 mentions) Hexokinase 2, by Abcam (1 mentions) Lactate dehydrogenase a, by Cell Signaling Technology (1 mentions)

2 protocols using stearoyl coa desaturase 1

1

Comprehensive Protein Extraction and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total proteins were extracted through 30-minute incubation on ice with Nonidet P-40 lysis buffer containing protease and phosphatase inhibitors, and resolved on Bolt Bis-Tris Plus polyacrylamide gels (Life Technologies). Antibodies against PARP (cat# 9532S), Phospho-Akt (S473; cat# 4060S), Akt (cat# 4685S), Phospho-Erk (T202/Y204; cat# 9101S), Erk1/2 (cat# 9102S), Phospho-S6 ribosomal protein (S235/236; cat# 2211S), total S6 ribosomal protein (cat# 2317S), Phospho-S6 kinase (cat# 9234S), total S6-kinase (cat# 2708S), Tuberin/TSC2 (cat# 4308S), Phospho-RSK (S380) (cat# 9335), total RSK (cat# 9355), Fatty Acid Synthase (cat# 3180S), Acetyl-CoA Carboxylase (cat# 3676S), Stearoyl-CoA desaturase 1 (cat# 2794S), CCTα (cat# 6931S) and BrdU (5292S) were obtained from Cell Signaling Technology (Danvers, MA). Anti-beta actin antibody (cat# A5316) was obtained from Millipore Sigma (St. Louis, MO) and anti-CPT1A antibody (cat# ab128568) from Abcam (Cambridge, MA).
Feng Y., Mischler W.J., Gurung A.C., Kavanagh T.R., Androsov G., Sadow P.M., Herbert Z.T, & Priolo C. (2020). Therapeutic targeting of the secreted lysophospholipase D autotaxin suppresses tuberous sclerosis complex-associated tumorigenesis. Cancer research, 80(13), 2751-2763.
+ Open protocol
+ Expand
2

Molecular Mechanisms of EMT and Metabolism

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant human TNFα and TGFβ were purchased from R&D Systems (Minneapolis, MN, USA). Antibodies used are listed as follows: IkB, phospho-Smad2/3, total-Smad2/3, Snail, vimentin, lactate dehydrogenase-A, fatty acid synthase, stearoyl-CoA desaturase-1, phospho-acetyl-CoA carboxylase, total-acetyl-CoA carboxylase (Cell signaling, MA, USA); beta-actin, E-cadherin, N-cadherin (Santa Cruz, TX, USA); hexokinase II, pyruvate kinase isoform 2, fructose-bisphophotase-2, total OXPHOS Human WB Antibody Cocktail (Abcam, Cambridge, UK); Zeb1 (ProSci, CA, USA); and fructose-bisphophotase-1 (Abgent, Wuxi, China). All other reagents were from Sigma-Aldrich (MO, USA) unless stated otherwise.
Liu M., Quek L.E., Sultani G, & Turner N. (2016). Epithelial-mesenchymal transition induction is associated with augmented glucose uptake and lactate production in pancreatic ductal adenocarcinoma. Cancer & Metabolism, 4, 19.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!