Proteins were separated on 10% SDS-PAGE gels and transferred to nitrocellulose membranes. Membranes were blocked with 5% bovine serum albumin and incubated with the indicated primary antibodies. Corresponding horseradish peroxidase-conjugated secondary antibodies were used following primary antibody incubation. The primary antibodies used were as follows: anti-HDAC2 (Proteintech, China), anti-Bax (Cell Signaling Technology, USA), anti-Bcl-2 (Cell Signaling Technology, USA), anti-CyclinD1 (Cell Signaling Technology, USA), anti-p21 (Cell Signaling Technology, USA), anti-Pan-acetylation (PTM Biolabs China), anti-C5aR (Abcam, USA), anti-PTEN (Proteintech, China), anti-AKT (Cell Signaling Technology, USA), anti-p-AKT (Cell Signaling Technology, USA), anti-GAPDH (Abclonal, China), and anti-β-actin (Abclonal, China).
Anti hdac2
Manufactured by Proteintech
Sourced in China
Anti-HDAC2 is a laboratory reagent that can be used to detect and quantify the HDAC2 protein. HDAC2 is a histone deacetylase enzyme that plays a role in regulating gene expression. The anti-HDAC2 reagent can be used in various laboratory techniques, such as Western blotting and immunoprecipitation, to study the expression and function of HDAC2 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti p akt, by Cell Signaling Technology (3 mentions)
Anti p21, by Proteintech (2 mentions)
Anti egfr viii, by Cell Signaling Technology (2 mentions)
Anti h2azk4 7ac, by Cell Signaling Technology (2 mentions)
Anti hdac1, by Proteintech (2 mentions)
Anti usp11, by Proteintech (2 mentions)
Anti cdk6, by ABclonal (2 mentions)
Anti h3k27ac, by Cell Signaling Technology (2 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti bcl 2, by Cell Signaling Technology (1 mentions)
Anti cyclin d1, by Cell Signaling Technology (1 mentions)
Anti c5ar, by Abcam (1 mentions)
Anti bax, by Cell Signaling Technology (1 mentions)
Anti β actin, by ABclonal (1 mentions)
Anti pten, by Proteintech (1 mentions)
Anti p21, by Cell Signaling Technology (1 mentions)
Anti gapdh, by ABclonal (1 mentions)
Anti h3, by Cell Signaling Technology (1 mentions)
Anti h2az, by Cell Signaling Technology (1 mentions)
Anti h3k4me3, by Cell Signaling Technology (1 mentions)
5 protocols using anti hdac2
1
Western Blot Analysis of Protein Signaling
2
Comprehensive Western Blotting Procedure
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Western blotting was carried out as described previously [31 (link)]. Anti-EGFRvIII, anti-P-AKT, anti-H3K27AC, anti-H2AZK4/7AC, anti-H3K4me3, anti-H3, anti-H2AZ (1:1000, Cell Signaling Technology), anti-HDAC1, anti-HDAC2, anti-USP11, anti-GAPDH, anti-P21 (1:1000, Proteintech), anti-CDK6 (1:1000, ABclonal), anti-CDK4 (1:500, AbSci) and anti-Cyclin D1 (1:500, Absin) primary antibodies were used.
3
Immunohistochemical Analysis of HDAC2 in Secondary Liver Cancer
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Secondary liver cancer samples were collected, embedded, and sectioned. Immunohistochemical staining was performed according to standard protocol, and the primary antibody was anti-HDAC2 (1:100, Proteintech). The antibody catalog in the Western blot and immunohistochemistry (IHC) analysis is 12922-3-AP (Proteintech Group Inc.). The staining scores were calculated using the immunoreactive score (IRS) system. The percentage of positive cells was scored as follows: no stained cells = 0; <10% staining = 1; 10%–50% staining = 2; 51%–80% staining = 3; and >80% staining = 4. The staining intensity was scored as follows: no color reaction = 0; mild reaction = 1; moderate reaction = 2; and intense reaction = 3. The IRS scores = (scores of staining intensity) × (scores of percentage of positive cells).
4
Immunohistochemistry Staining for Glioblastoma
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H&E and IHC staining was performed as previously described [12 (link)]. The following primary antibodies were used: anti-EGFR-vIII (1:200, Cell Signaling Technology), anti-P-AKT (1:100, Cell Signaling Technology), anti-H3K27AC (1:50, Cell Signaling Technology), anti-H2AZK4/7AC (1:50, Cell Signaling Technology), anti-HDAC1 (1:100, Proteintech), anti-HDAC2 (1:100, Proteintech), anti-USP11 (1:50, Proteintech), anti-P21 (1:400, Proteintech), anti-CDK6 (1:100, ABclonal), and anti-CDK4 (1:100, AbSci).
5
Formalin-Fixed Tissue Analysis
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Tumor tissue from mice was fixed with formalin; then H&E staining was performed following the manufacturer’s instructions. IHC was performed using anti-Ki-67 (Proteintech, China) and anti-HDAC2 (Proteintech, China) antibodies.
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