Egg yolk phospholipids

Manufactured by Merck Group

Sourced in United States, Italy

Egg yolk phospholipids are a class of naturally occurring lipids derived from egg yolks. They are composed of phospholipids, which are the main structural components of cell membranes. Egg yolk phospholipids serve as a source of these essential lipids for various applications in the laboratory setting.

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Phospholipids (7 citations)

5 protocols using egg yolk phospholipids

Radiolabeled Acetylcholine Binding Assay

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Amberlite XAD-4, egg yolk phospholipids (3-sn-phosphatidylcoline 60%), CHS, Triton-X100, Sephadex G-75, TEA and acetylcholine were purchased from Merck Life Science s.r.l., 20149 Milano, Italy; tetraethylammonium bromide [ethyl-1-¹⁴C] was from ARC (American Radiolabeled Chemicals, St. Louis, MO 63146 USA); acetylcholine iodide [acetyl-³H] was from Perkin-Elmer Italia S.p.A. 20126 Milano, MI, Italy. All the other reagents were of analytical grade.

Pochini L., Pappacoda G., Galluccio M., Pastore F., Scalise M, & Indiveri C. (2020). Effect of Cholesterol on the Organic Cation Transporter OCTN1 (SLC22A4). International Journal of Molecular Sciences, 21(3), 1091.

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Purification and Characterization of Membrane Proteins

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E. coli Rosetta(DE3)pLysS cells were from Novagen (Rome, Italy); His Trap HP and PD10 columns were from GE Healthcare; l-[³H]histidine was from American Radiolabeled Chemicals (ARC Inc., USA); C₁₂E₈, Amberlite XAD-4, egg yolk phospholipids (3-sn-phosphatidylcholine from egg yolk), cholesterol, Sephadex G-75, imidazole, l-histidine and all the other reagents were from Merck KGaA (Germany).

Cosco J., Scalise M., Colas C., Galluccio M., Martini R., Rovella F., Mazza T., Ecker G.F, & Indiveri C. (2020). ATP modulates SLC7A5 (LAT1) synergistically with cholesterol. Scientific Reports, 10, 16738.

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Radioisotope Labelling of Carnitine and Ornithine

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L-[methyl-³H]carnitine and L-[2,3-³H]ornithine from Scopus Research BV Costerweg, Sephadex G-75, egg-yolk phospholipids (l-α phosphatidylcholine from fresh turkey egg yolk), PIPES, HEPES, Triton X-100, cardiolipin, L-carnitine, L-ornithine, sodium itaconate, dimethyl itaconate (DMI), N-ethylmaleimide (NEM) were purchased from Sigma-Aldrich, Milan, Italy. All other reagents were of analytical grade.

Giangregorio N., Tonazzi A., Console L., Scalise M, & Indiveri C. (2023). Inhibition of the Mitochondrial Carnitine/Acylcarnitine Carrier by Itaconate through Irreversible Binding to Cysteine 136: Possible Pathophysiological Implications. Biomolecules, 13(6), 993.

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Purification of His-Tagged Proteins

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E. coli Rosetta(DE3)pLysS cells were from Novagen (Rome, Italy); ECL plus, Hybond ECL membranes were from GE Healthcare; l-[³H]Arg was from Perkin Elmer (Waltham, MA, USA); conjugated anti-His6 antibody, TX-100, Amberlite XAD-4, egg yolk phospholipids (3-sn-phosphatidylcholine from egg yolk), Sephadex G-75, His-Select resin, l-Arg and all the other reagents were from Sigma-Aldrich (Saint Louis, MO, USA).

Cosco J., Regina T.M., Scalise M., Galluccio M, & Indiveri C. (2019). Regulatory Aspects of the Vacuolar CAT2 Arginine Transporter of S. lycopersicum: Role of Osmotic Pressure and Cations. International Journal of Molecular Sciences, 20(4), 906.

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Telmisartan Lipid-Based Nanoformulation

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Telmisartan was received as a free sample from International Drug agency for pharmaceutical Industry (IDI) (Egypt). Maltodextrin, span 40 and cholesterol were purchased from S.D. Fine Chemicals (Mumbai, India). Brij 35 and egg yolk phospholipids were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Soya bean phospholipids were a free sample from Lipoid (Germany). Chloroform and all other chemicals were obtained from El-Nasr Pharmaceutical Chemical Co. (Cairo, Egypt). Avicel was a free sample compassionately provided by the Egyptian International Pharmaceutical Industries Co., EIPICO (Egypt). All materials utilized as a part of the examination were of systematic review and utilized without further filtration.

Teaima M.H., Yasser M., El-Nabarawi M.A, & Helal D.A. (2020). Proniosomal Telmisartan Tablets: Formulation, in vitro Evaluation and in vivo Comparative Pharmacokinetic Study in Rabbits. Drug Design, Development and Therapy, 14, 1319-1331.

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