For immunofluorescence, tissue sections were incubated with the following primary antibodies: mouse anti-α-smooth muscle actin (α-SMA; 1:5000; Abcam, UK), rabbit anti-collagen Ⅳ (Col Ⅳ; 1:200; Abcam, UK), mouse anti-laminin (LN; 1:200; Abcam, UK). After being washed in PBS, sections were then incubated in 488- or 594-conjugated species-specific secondary antibody (1:1000; Chemicon, USA) for 2 h at room temperature. Slides were observed with an Olympus fluorescent microscope and images were captured using Olympus image viewer.
Rabbit anti collagen 4 col 4
Manufactured by Abcam
Sourced in United Kingdom
Rabbit anti-collagen IV (Col IV) is a primary antibody that recognizes collagen type IV, a major structural component of basement membranes. It can be used for detecting and quantifying collagen IV in various applications such as immunohistochemistry, western blotting, and ELISA.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti α smooth muscle actin α sma, by Abcam (2 mentions)
Mouse anti laminin ln, by Abcam (2 mentions)
Fluorescent microscope, by Olympus (1 mentions)
Image viewer, by Olympus (1 mentions)
488 or 594 conjugated species specific secondary antibody, by Merck Group (1 mentions)
α sma, by Leica (1 mentions)
2 protocols using rabbit anti collagen 4 col 4
1
Immunofluorescence Staining of Tissue Sections
2
Immunohistochemical Analysis of Extracellular Matrix
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For immunohistochemical staining, tissue sections were incubated with the following antibodies: mouse anti-α-smooth muscle actin (α-SMA; 0.034 μg/ml; Abcam, UK), rabbit anti-collagen Ⅳ (Col Ⅳ; 1:2000; Abcam, UK), mouse anti-laminin (LN; 1:2000; Abcam, UK).The stained sections were scanned on a microscope (Leica Microsystems, Wetzlar, Germany).
Areal density was used for analysis of α-SMA, Col IV and LN. Five images were randomly taken for analysis in one section of each animal. The brown yellow was used as the standard to define the positive staining via the Image J software. The pixel area were measured in each photo.
Areal density was used for analysis of α-SMA, Col IV and LN. Five images were randomly taken for analysis in one section of each animal. The brown yellow was used as the standard to define the positive staining via the Image J software. The pixel area were measured in each photo.
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