Paraffin-embedded tissue sections (4 μm) were subjected to antigen retrieval, incubation with anti-CD31 (ab28364, abcam), anti-VEGF-A (BA0407, Boster), anti-HIF-1A (PB0245, Boster), anti-CD34 (BA0532, Boster), or anti-TSP1 (ab93653, abcam) antibodies, followed by incubation with a peroxidase-conjugated goat anti-rabbit IgG (KIT-9706, Maixin-Bio) secondary antibody. Samples were developed with DAB or AEC and counterstained with Mayor's hematoxylin (AR0005, Boster). Image Pro Plus 6.0 software was used for quantitative analysis.
Pb0245
Manufactured by Boster Bio
Sourced in United States
PB0245 is a laboratory centrifuge designed for general-purpose applications. It is capable of processing a variety of sample types and volumes. The centrifuge features a compact and durable construction, making it suitable for use in various laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Ba0407, by Boster Bio (2 mentions)
Ba3394, by Boster Bio (2 mentions)
Pb0343, by Boster Bio (2 mentions)
Pb0174, by Boster Bio (2 mentions)
Ab28364, by Abcam (1 mentions)
Ba0532, by Boster Bio (1 mentions)
Kit 9706, by Maixin Group (1 mentions)
Ar0005, by Boster Bio (1 mentions)
Rc 5g5, by Kangchen Biotech (1 mentions)
Ba1054, by Boster Bio (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Inverted fluorescence microscope, by Olympus (1 mentions)
Goat anti rabbit igg antibody, by Boster Bio (1 mentions)
Ba1003, by Boster Bio (1 mentions)
4 protocols using pb0245
1
Immunohistochemical Analysis of Angiogenic Factors
2
Western Blot Analysis of Apoptosis Regulators
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Western blotting was performed using primary antibodies against RIP1 (1:1,000, 17519-1-AP; Proteintech, Rosemont, IL, USA), RIP3 (1:1,000, 17563-1-AP; Proteintech), HIF-1α (1:1,000, PB0245; BOSTER Biological Technology, Pleasanton, CA, USA), TRPC6 (1:400, BA3394; BOSTER Biological Technology), PARP1 (1:1,000, PB0343; BOSTER Biological Technology), Sirtuin-2 (1:1,000; PB0174; BOSTER Biological Technology), and GAPDH (1:10,000, RC-5G5; KangChen Bio-tech, Shanghai, China), followed by incubation with a horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (IgG) antibody (1:20,000, BA1054; BOSTER Biological Technology) for 1 h at room temperature. The signals were detected by enhanced chemiluminescence (Pierce, Rockford, IL, USA).
3
Immunofluorescence Staining of HK-2 Cells
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For immunofluorescence staining, cultured HK-2 cells were fixed with 4% buffered paraformaldehyde in PBS and then washed with PBS for 5 min. Next, the cells were incubated overnight at 4°C with primary antibodies against RIP3 (17563-1-AP; Proteintech), HIF-1α (PB0245; BOSTER Biological Technology), TRPC6 (BA3394; BOSTER Biological Technology), PARP1 (PB0343; BOSTER Biological Technology), and Sirtuin-2 (PB0174; BOSTER Biological Technology), followed by incubation with a FITC-labeled anti-goat secondary antibody (Beyotime Institute of Biotechnology). The cell nuclei were stained with DAPI (Beyotime, Shanghai, China) for 20 min at room temperature. Immunofluorescence was examined under an inverted fluorescence microscope (Olympus Corporation, Japan).
4
OSCC Tissue Immunohistochemistry Analysis
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All the OSCC specimens were obtained from the 73 patients. Ten corresponding normal tissue samples, which came from the 73 patients' normal oesophagus (5 cm away from ESCC) randomly, were used as controls. The tissue specimens were fixed in 10% neutral buffered formalin and processed routinely. Hypoxiainducible factor 1α and VEGF were detected by the streptavidin-peroxidase (SP) method using the same paraffin-embedded tissue samples, which were cut into 4-mm-thick slices. The primary antibody was applied using rabbit anti-human monoclonal HIF-1α antibodies (1:100, Catalogue #PB0245, Boster Biological Technology, Wuhan, P.R. China) or rabbit anti-human monoclonal VEGF antibodies (1:100, Catalogue #BA0407, Boster Biological Technology, Wuhan, P.R. China). A secondary antibody was applied using Goat Anti-Rabbit IgG Antibody (1:100, Catalogue #BA1003, Boster Biological Technology, Wuhan, P.R. China). When intensive positive staining of HIF-1α or VEGF was observed in more than 10% of the tumour cells, the case was considered HIF-1αpositive or VEGF-positive. These evaluation criteria have been described in previous reports [8, 9, 10, 11] .
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