Horseradish peroxidase hrp conjugated goat anti rabbit or mouse secondary antibodies

Bicinchoninic acid (BCA) protein assay kit (P0009), LDH cytotoxicity assay kit (C0016), enhanced chemiluminescence (ECL) kit (P0018M), MTT cell proliferation and cytotoxicity assay kit (C0009), Hoechst staining kit (C0003), benzyloxycarbonyl-Val-Ala-Asp fluoromethylketone (Z-VAD) (C1202), were obtained from Beyotime Institute of Biotechnology (Haimen, Jiangsu, China). Glutathione (GSH) assay kit was obtained from Nanjing Jiancheng Bioengineering Institute (Jiancheng, Nanjing, Jiangsu, China). Hydrogen peroxide (H₂O₂) and 3-methyladenine (3-MA) were obtained from Sigma-Aldrich (St. Louis, USA). Rabbit monoclonal anti-caspase-3 (cleaved) antibody was obtained from Beyotime Institute of Biotechnology. Rabbit polyclonal anti-LC3B antibody and horseradish peroxidase (HRP)-conjugated goat anti-rabbit or -mouse secondary antibodies were purchased from Sigma-Aldrich. Mouse monoclonal anti-β-actin antibody and rabbit polyclonal anti-ATG5 antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, USA). X-tremeGENE siRNA transfection regent was from Roche (Basel, Switzerland).

Rapamycin (R0935), rabbit polyclonal anti-LC3B antibody (L7543) and horseradish peroxidase (HRP)-conjugated goat anti-rabbit or -mouse secondary antibodies were purchased from Sigma-Aldrich (St. Louis, USA). Rabbit polyclonal anti-ATG5 antibody (sc-33210) and mouse monoclonal anti-β-actin antibody (sc-47778) were purchased from Santa Cruz Biotechnology (Santa Cruz, USA). Rabbit anti-p-AKT (Ser473) antibody (4060) and anti-p-mTOR antibody (Ser2448) (5536) were purchased from Cell signaling Technology (Beverly, USA). Rabbit anti-AKT antibody (ab32505) and anti-mTOR antibody (ab2732) were purchased from Abcam (Cambridge, UK). 4′, 6-diamidino-2-phenylindole (DAPI) (C1005) and insulin (P3376) were purchased from Beyotime Biotechnology (Haimen, China).