Vismodegib

Manufactured by MedChemExpress

Sourced in United States

Vismodegib is a lab equipment product that is used for research purposes. It functions as a small molecule inhibitor of the Hedgehog signaling pathway, which is involved in cellular development and growth. The core function of Vismodegib is to inhibit the Hedgehog pathway, but its specific applications and intended use should not be extrapolated beyond this factual description.

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Lab products found in correlation

Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (2 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (2 mentions) Gapdh, by Proteintech (2 mentions) Actinomycin d acd, by MedChemExpress (2 mentions) Rabbit anti gli1 antibody, by Beyotime (2 mentions) Mouse anti gapdh antibody, by Beyotime (2 mentions) Genistein, by Merck Group (1 mentions) Cleaved caspase 3, by Proteintech (1 mentions) Insulin, by Thermo Fisher Scientific (1 mentions) Purmorphamine, by MedChemExpress (1 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions) Cleaved caspase 8, by Cell Signaling Technology (1 mentions) Cleaved caspase 9, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Venetoclax, by MedChemExpress (1 mentions) Daunorubicin, by Selleck Chemicals (1 mentions) Cladribine, by Bio-Techne (1 mentions) Gedatolisib, by Selleck Chemicals (1 mentions) Clofarabine, by Bio-Techne (1 mentions) Volasertib, by Selleck Chemicals (1 mentions)

8 protocols using vismodegib

Investigating Hedgehog Signaling Modulators

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Genistein and DMSO were purchased from Sigma-Aldrich; Merck KGaA. Vismodegib and purmorphamine were purchased from MedChemExpress and dissolved in DMSO at a stock solution concentration of 10 mM. Epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and insulin were purchased from PeproTech, Inc.. In addition, 2% B27 was acquired from Gibco; Thermo Fisher Scientific, Inc..
The primary antibodies that were used in the present study, including Shh (cat. no. 20697-1-AP), Gli1 (cat. no. 66905-1-Ig), Gli2 (cat. no. 18989-1-AP), CD44 (cat. no. 15675-1-AP), CD133 (cat. no. 18470-1-AP), aldehyde dehydrogenase 1 family member A1 (ALDH1A1) (cat. no. 15910-1-AP), Oct4 (cat. no. 11263-1-AP), Nanog (cat. no. 14295-1-AP), cyclin D1 (cat. no. 60186-1-Ig), proliferating cell nuclear antigen (PCNA) (cat. no. 10205-2-AP), Bcl-2 (cat. no. 12789-1-AP), Bax (cat. no. 50599-2-Ig), and GAPDH (cat. no. 10494-1-AP) were purchased from ProteinTech, Inc.. Cleaved caspase 3 (cat. no. 9661), cleaved caspase 8 (cat. no. 8592) and cleaved caspase 9 (cat. no. 20750) were obtained from Cell Signaling Technology, Inc.. Smo (cat. no. DF5152) was obtained from Affinity Biosciences, Inc. Anti-rabbit (cat. no. SA00001-2) and anti-mouse (cat. no. SA00001-1) second antibodies were purchased from ProteinTech, Inc..

Li E., Zhang T., Sun X., Li Y., Geng H., Yu D, & Zhong C. (2019). Sonic hedgehog pathway mediates genistein inhibition of renal cancer stem cells. Oncology Letters, 18(3), 3081-3091.

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Evaluation of nucleoside analogs

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CNDAC was purchased from biorbyt (via Biozol, Eching, Germany), 5-azacytidine, cytarabine, cladribine, clofarabine, decitabine, and fludarabine from Tocris Biosciences (via Bio-Techne GmbH, Wiesbaden, Germany), 6-thioguanine, ganetespib, molibresib, olaparib, sapacitabine, venetoclax, and vismodegib from MedChemExpress (via Hycultec, Beutelsbach, Germany), daunorubicin, gedatolisib, and volasertib from Selleckchem (Berlin, Germany), gemcitabine from Hexal (Holzkirchen, Germany), GTP and dATP from Thermo Scientific (Dreieich, Germany), and CNDAC-TP from Jena Bioscience GmbH (Jena, Germany).

Rothenburger T., Thomas D., Schreiber Y., Wratil P.R., Pflantz T., Knecht K., Digianantonio K., Temple J., Schneider C., Baldauf H.M., McLaughlin K.M., Rothweiler F., Bilen B., Farmand S., Bojkova D., Costa R., Ferreirós N., Geisslinger G., Oellerich T., Xiong Y., Keppler O.T., Wass M.N., Michaelis M, & Cinatl J J.r. (2021). Differences between intrinsic and acquired nucleoside analogue resistance in acute myeloid leukaemia cells. Journal of Experimental & Clinical Cancer Research : CR, 40, 317.

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Kidney Cancer Cell Proliferation Assay

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Human kidney cancer cell lines 786-O and ACHN were purchased from Chinese Academy of Typical Culture Collection Cell Bank (Shanghai, China), cultured in 37 °C, 5% CO₂, and saturated humidity. 786-O cells were maintained in RPMI 1640 (Gibco, Carlsbad, CA, USA) medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. ACHN cells grew in the same condition except in MEM (Gibco, Carlsbad, CA, USA) medium. Vismodegib was acquired from Medchem Express (NJ, USA). Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) were acquired from Peprotech (Rocky Hill, NJ, USA). Insulin and 2% B27 were acquired from Gibco. Primary antibodies including CD133, CD44, ALDHA1, Oct4, Nanog, PCNA, CyclinD1, Shh, Smo, Gli1, Gli2, and GAPDH were purchased from Proteintech (Rocky Hill, NJ, USA). Anti-rabbit and anti-mouse second antibodies were purchased from ZSGB-BIO (Beijing, China). PCR primers of CD133, CD44, ALDHA1, Oct4, and Nanog were synthesized by Beijing Genomics Institute (Beijing, China).

Qian W., Kong X., Zhang T., Wang D., Song J., Li Y., Li X., Geng H., Min J., Kong Q., Liu J., Liu Z., Wang D., Zhang Z., Yu D, & Zhong C. (2018). Cigarette smoke stimulates the stemness of renal cancer stem cells via Sonic Hedgehog pathway. Oncogenesis, 7(3), 24.

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Comprehensive Chemical Compound Library

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Emetine (HY–B1479A, 99.81%, LCMS), salinomycin (HY-15597, >98%, NMR), tilorone (HY–B1080, 99.9%, LCMS), chloroquine (HY-17589, 99.9%, LCMS), homoharringtonine (HY-14944, 99.2%, LCMS), gemcitabine (HY–B0003, 99.9%, LCMS), vismodegib (HY-10440, 99.9%, LCMS), conivaptan (HY-18347A, 99.9%, LCMS), and atovaquone (HY-13832, 99.8%, LCMS) were purchased from MedChem Express (Monmouth Junction, NJ, USA); niclosamide (S3030, 99.8%, HPLC) and nitazoxanide (S1627, 99.3%, HPLC) were from Selleckchem (Houston, TX, USA); antimycin A (A8674, 97.64%, HPLC), anisomycin (A9789, >98%, HPLC) oligomycin (O4876, >90%, HPLC), valinomycin (V0627, ≧90%, HPLC) and crystal violet (C0775, Dye content ≥90%) were from Sigma–Aldrich (St. Louis, MO, USA); GS-441524 (AG167808, >98%, HPLC) were from Carbosynth (San Diego, CA, USA). All chemicals were used as supplied.

Yang C.W., Peng T.T., Hsu H.Y., Lee Y.Z., Wu S.H., Lin W.H., Ke Y.Y., Hsu T.A., Yeh T.K., Huang W.Z., Lin J.H., Sytwu H.K., Chen C.T, & Lee S.J. (2020). Repurposing old drugs as antiviral agents for coronaviruses. Biomedical Journal, 43(4), 368-374.

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Targeting Stroma in Organoid Cultures

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For targeting the stroma, several compounds were added as 2 µM solutions to the OrganoPlate after medium was removed on day 4. The following compounds were used: Halofuginone (MedChem Express, #HY-N1584) as a PSC/CAF and SMAD 2/3 inhibitor; Galunisertib (Selleck Chemicals, #S2230) as TGF-β receptor inhibitor; Vismodegib (MedChem Express, #HY-10440) as a SHH pathway inhibitor, Pirfenidone (MedChem Express, #HY-B0673) as a cell cycle inhibitor of CAFs; and Losartan (MedChem Express, #HY-17512) as a TGF-β ligand inhibitor (16 (link)).

Geyer M., Gaul L.M., D`Agosto S.L., Corbo V, & Queiroz K. (2023). The tumor stroma influences immune cell distribution and recruitment in a PDAC-on-a-chip model. Frontiers in Immunology, 14, 1155085.

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Vismodegib Dose-Response in Xenopus Embryos

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A 1 mM stock solution of Vismodegib (MedChem Express; catalog # HY-10440) was made in DMSO. Once the embryos were confirmed to be undergoing gastrulation (Koebernick et al., 2003 (link)), twenty embryos each were treated with 6.25 μM, 12.5 μM, 18.75 μM, and 21.875 μM Vismodegib. An additional group of twenty was left untreated (control animals). Each group was kept at 15 – 18 °C in 30 mm Petri dishes until they reached stage 46 (Nieuwkoop and Faber, 1956 ). The Vismodegib solution was replaced daily to ensure consistency of the chosen concentration over time. This interval is consistent with Vismodegib’s half-life, which is suggested to be 14 hours in humans and over two days of constant plasma levels (Rudin, 2012 (link)). What the exact concentration of IC₅₀ is for Xenopus Smo proteins is unknown.

Zarei S., Zarei K., Fritzsch B, & Elliott K.L. (2017). Sonic hedgehog antagonists reduce size and alter patterning of the frog inner ear. Developmental neurobiology, 77(12), 1385-1400.

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Isolation and Characterization of Toosendan Saponin

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TSN was isolated from the bark of Melia toosendan Sieb. et Zucc. and identi ed by NMR spectroscopy. The purity of TSN was determined to be more than 99% by HPLC. Vismodegib was purchased from MedChemExpress (USA), actinomycin D (ACD) was purchased from MedChemExpress (USA), the mouse anti-SHH antibody (E-1, sc-365112) and mouse anti-SMO antibody (E-5, sc-166685) were purchased from Santa Cruz (USA), and the rabbit anti-GLI1 antibody and mouse anti-GAPDH antibody were purchased from Beyotime (Beijing).

Zhang M., Tao Z., Gao L., Chen F., Ye Y., Xu S., Huang W, & Li X. (2022). Toosendanin inhibits colorectal cancer cell growth through the Hedgehog pathway by targeting Shh. Drug development research, 83(5).

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Isolation and Characterization of Toosendan Saponin

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Zhang M., Tao Z., Gao L., Chen F., Ye Y., Xu S., Huang W., & Li X. (2021). Toosendanin Inhibits Colorectal Cancer Cell Growth Through the Hedgehog Pathway by Targeting SHH.

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