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Cd90.2 buv395

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CD90.2 BUV395 is a fluorochrome-conjugated anti-mouse CD90.2 (Thy-1.2) antibody. CD90.2 is a cell surface glycoprotein expressed on a variety of cell types, including T cells, hematopoietic stem cells, and neurons. The BUV395 fluorochrome allows for detection and analysis of CD90.2-positive cells using flow cytometry.

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Lab products found in correlation

Cd44 fitc, by BioLegend (1 mentions) Cd8 fitc, by BioLegend (1 mentions) Lag3 bv785, by BioLegend (1 mentions) Cd62l bv510, by BioLegend (1 mentions) Tim3 apc fire750, by BioLegend (1 mentions) Cd4 buv496, by BD (1 mentions) Nk1.1buv661, by BD (1 mentions) Cd8 buv805, by BD (1 mentions) Cd62l bv605, by BD (1 mentions) Cd4 apc cy7, by BioLegend (1 mentions) B220 apc, by BioLegend (1 mentions) Nk1.1 apc, by BioLegend (1 mentions) Cd11c apc, by BioLegend (1 mentions) Cd44 bv650, by BioLegend (1 mentions) Cd8 percp cy5, by BioLegend (1 mentions) Cd4 buv737, by BD (1 mentions) Cd11b apc, by BioLegend (1 mentions) Cd11b bv421, by BioLegend (1 mentions) Cd8a buv 737, by BD (1 mentions) Gr1 af 647, by BioLegend (1 mentions)

3 protocols using cd90.2 buv395

1

Multiparameter Flow Cytometry for Immune Cell Analysis

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Fluorochrome-conjugated antibodies were used to select and sort cell subsets, analyze T cell phenotypes, and to determine cytokine production. The following antibodies were used in this study, separated by manufacturer (clone is indicated in parentheses). Biolegend: Ki67-PacificBlue (16A8), CD62L-BV510 (MEL-14), CD73-BV605 (TY/11.8), CD44-FITC (IM7), PD1-PEDazzle594 (RMP1–30), TIGIT-PECy7 (1G9), LAG3-BV785 (C9B7W), IFNγ (XMG1.2), TNFα-PECy7 (MP6-XT22), SATB1-AlexaFluor594 (O96C6), TIM3-APC/Fire750 (B8.2C12), OX40-BV711 (OX-86), OX40L-PECy7 (RM134L), Tim3-PerCP/Cy5.5 (B8.2C12), CD8-FITC (53–6.7), CD90.2-PECy7 (30H12), CD90.2-PerCP/Cy5 (53–2.1), CD4-APCCy7 (RM4–5). || BD Biosciences: CD90.2-BUV395 (53–2.1), CD4-BUV496 (GK1.5), CD19-BUV661 (1D3), CD11c-BUV661 (N418), F4/80-BUV661 (T45–2342), NK1.1BUV661 (PK136), TER119-BUV661 (TER-119), CD127-BUV737 (SB/199), CD8-BUV805 (53–6.7), FR4-BV421 (12A5), CTLA4-APCR700 (UC10–4F10–11), NK1.1-eFluor450 (PK136), Ter-119-eFluor450 (Ter119), Rorγt-BV650 (Q31–378), CD62L-BV605 (MEL-14). || Invitrogen: FoxP3-AlexaFluor532 (FJK-16s), CD44-BUV737 (IM7), PD1-SB780 (J43), TOX-eFluor660 (TXRX10), EOMES-PerCP/eFluor710 (Dan11mag), F4/80-eFluor450 (BM8), CD49b-eFluor450 (DX5), CD11c-eFluor450 (N418), PD1-PerCPe710 (J43). || Santa Cruz Biotechnologies: NFATc1-AlexaFluor488 (7A6), CD30L-AlexaFluor680 (RM153).
Pollard J., Hynes G., Yin D., Mandal M., Gounari F., Alegre M.L, & Chong A. (2023). Pregnancy programs epigenetic and transcriptional exhaustion in memory CD8+ T cells. Research Square.
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2

Multiparametric Flow Cytometry Panel

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CD90.1 BB515 (BD, clone OX-7, cat 564607), CD90.2 BUV395 (BD, clone 53-2-1, cat 565257), CD4 BUV737 (BD, clone RM4-5, cat 565246), CD8 PerCP-Cy5.5 (Biolegend, clone 53–6.7, cat 100734), CD44 BV650 (Biolegend, clone IM7, cat 1033049), CD11b APC (Biolegend, clone M1/70, cat 101212), CD11c APC (Biolegend, clone N418, cat 117310), NK1.1 APC (Biolegend, clone PK136, cat 108710), B220 APC (Biolegend, clone RA3-62B, cat 103212).
dos Santos Dias L., Dobson H.E., Bakke B.K., Kujoth G.C., Huang J., Kohn E.M., Taira C.L., Wang H., Supekar N.T., Fites J.S., Gates D., Gomez C.L., Specht C.A., Levitz S.M., Azadi P., Li L., Suresh M., Klein B.S, & Wüthrich M. (2021). Structural basis of Blastomyces Endoglucanase-2 adjuvancy in anti-fungal and -viral immunity. PLoS Pathogens, 17(3), e1009324.
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3

Skin and Lymph Node Immune Cell Profiling

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Single cell suspensions were obtained from uninfected skin and lymph nodes as previously described [13] (link). Intracellular CD207 staining was performed using BD Bioscience Cytofix/Cytoperm kit (BD Biosciences, San Jose, CA). For extracellular staining, antibodies were diluted in staining media (3% Calf Serum, 5mM EDTA, 0.04% Azide) with Fb block. The following antibodies were used for skin: CD45.2 AF488, CD64 PE, TCRβ PE-Dazzle 594, CD3ε PE-Cy7, Gr1 AF 647, MHCII AF 700, CD11b BV421, TCRγδ BV510 (all Biolegend, SanDiego, CA), CD11c PerCPCy5.5 (Tonbo Biosciences, San Diego, CA), CD90.2 BUV395, CD8a BUV737 (both BD Biosciences, San Jose, CA). For lymph node staining we used: CD45.2 AF488, CD64 PE, Gr1 AF 647, MHCII AF 700, CD11b BV421, (all Biolegend, SanDiego, CA), CD11c PerCPCy5.5 (Tonbo Biosciences, San Diego, CA). Sample data was acquired on an LSRFortessa flow cytometer (Becton Dickinson, Franklin Lakes, NJ), and analysed using FlowJo software (TreeStar, Ashland, OR).
Edwards T.N., Zhang S., Liu A., Cohen J., Zhou P.Y., Mogavero S., Hube B., Berman J., Bougnoux M., Mathers A.R., Gaffen S.L., Albers K.M., Koerber H.R., Davis B.M., d’Enfert C., & Kaplan D.H. (2020). Extracellular ATP released from Candida albicans activates non-peptidergic neurons to augment host defense.
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