αcd3 biotin

Manufactured by BioLegend

Sourced in United States

αCD3-Biotin is a biotinylated antibody that recognizes the CD3 complex on T cells. This antibody can be used for the detection and purification of T cells in various experimental applications.

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2 protocols using αcd3 biotin

Multiparametric Flow Cytometry Immunophenotyping

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Flow cytometry staining was performed with panels of antibodies specific for activated/memory T cells (αCD3-Biotin, αCD4-FITC, αCD8-APCeF780, αCD62L-PeCy7, αCD44-APC; all from BioLegend) in PBS with 0.5% FCS 2 mM EDTA for 15 min. For intracellular staining cells will be stimulated with a mix containing 10 µg/ml Brefeldin A (Sigma), 50 ng/ml PDBu (Sigma) and 500 ng/ml ionomycin (Sigma) in RPMI-1640 (PAN Biotech) plus 10% FCS (Biochrom) plus 1% penicillin/streptomycin (Lonza) plus 2 mM L-glutamine (Lonza) for 4 h. The cells were then formalin-fixed, permeabilized (0.05% Triton X-100 in PBS) and stained for cytokines (αIL-2-PE, αIFNγ-PeCy7), and transcription factors (αFOXP3-FITC) or perforin (αPerforin-APC) for 1 h. All antibodies were from Biolegend. Cells were analyzed with Gallios and Cytoflex S flow cytometers (Beckman Coulter) and FlowJo Software (Beckton Dickinson).

Tymoszuk P., Nairz M., Brigo N., Petzer V., Heeke S., Kircher B., Hermann-Kleiter N., Klepsch V., Theurl I., Weiss G, & Pfeifhofer-Obermair C. (2020). Iron Supplementation Interferes With Immune Therapy of Murine Mammary Carcinoma by Inhibiting Anti-Tumor T Cell Function. Frontiers in Oncology, 10, 584477.

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Comprehensive Immune Cell Profiling

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αCD2-biotin (clone: RPA-2.10), αCD14-biotin (clone: HCD14), αCD16-biotin (clone: 3G8), αCD19-biotin (clone: 561), αCD235a-biotin (clone: HIR2), αCD3-biotin (clone: OKT3a), αCD34-APC (clone: 561), αCD38-PE/Cy7 (clone: HIT2), αCD4-APC/Cy7 (clone: RPA-T4), αCD8a-Pacific Blue (clone: RPA-T8), αCD90-PerCP/Cy5.5 (clone: 5E10), αCD272-FITC (αBTLA; clone: MIH26), αIgG2-FITC (clone: MOPC-173), αCD200R-PE (clone: OX-108), αIgG1-PE (clone: MOPC-21), αCD160-PerCP/Cy5.5 (clone: BY55), αIgM-PerCP/Cy5.5 (clone: MM-30), αCD96-APC (clone: NK92.39), αIgG1-APC (MOPC-21), αCD28-PE/Cy7 (clone: CD28.2), αIgG1-PE/Cy7 (MOPC-21) and αStreptavidin-FITC were from BioLegend, USA. αCD45-PE (clone: HI30) was from eBioscience, USA. αIgG1-PE (clone: IS11-12E4.23.20) was from Miltenyi Biotec, Germany. Samples were analyzed on BD LSRFortessa™ (BD Biosciences, USA). Data was analyzed using FlowJo software v.10.7 (FlowJo, LLC, USA).

Radpour R., Stucki M., Riether C, & Ochsenbein A.F. (2021). Epigenetic Silencing of Immune-Checkpoint Receptors in Bone Marrow- Infiltrating T Cells in Acute Myeloid Leukemia. Frontiers in Oncology, 11, 663406.

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