A MaxiSorp 96-well plate was coated with a 50 μg/mL solution of purified antibody fragments in TBS, 50 μL per well, and incubated overnight at 4˚C. The wells were washed and blocked as above. The blocking solution was removed and the wells were incubated with serial dilutions of biotinylated htt peptide (MATLEKLMKAFESLKSFK(Biotin)-NH2; New England Peptide) in TBS for 1 h. The wells were washed 3x with TBST, then incubated with a 1:10,000 dilution of HRP-conjugated streptavidin (BioLegend) in TBS for 1 h. The wells were washed 3x with TBST, then developed, quenched, and absorbance read as above.
Matleklmkafeslksfk biotin nh2
Manufactured by Biosynth
MATLEKLMKAFESLKSFK(Biotin)-NH2 is a synthetic peptide compound produced by Biosynth. It is a custom-made product, and its core function is as a chemical tool for research and development purposes. The product specification includes the amino acid sequence and the presence of a biotin moiety attached to the N-terminus.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using matleklmkafeslksfk biotin nh2
1
Biotinylated Huntingtin Peptide Binding Assay
2
Biotinylated Huntingtin Peptide Binding Assay
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