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Eber probe

Manufactured by Agilent Technologies
Sourced in United States

The EBER probe is a laboratory equipment used for the detection of Epstein-Barr virus (EBV) encoded RNA (EBER) in tissue samples. The EBER probe is a nucleic acid probe that binds to the EBER sequences present in EBV-infected cells, allowing for the identification of EBV infection through in situ hybridization techniques.

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4 protocols using eber probe

1

EBER In-Situ Hybridization for EBV Detection

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To examine the EBV infection status, we stained 3‐μm‐thick sections for in situ hybridization. EBER probe (#Y5200; Dako) was detected using the PNA ISH Detection Kit (#K5201; Dako). Identifiable nuclear staining for EBER was interpreted as a positive result.
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2

EBV Detection in NKTCL Tissues

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We detected the presence of EBV in NKTCL tissues by measuring EBV encoded small RNA (EBER) using ISH method. Slices were incubated with fluorescein-labeled EBER probe (Dako, Santa Clara, USA) and stained on an automated IHC and ISH systems Bond-III (Leica, Wetzlar, Germany) using Bond Polymer RedRefine Detection Kit (Leica, Wetzlar, Germany). Images were captured by Nikon DS-U3 imaging system (Nikon, Tokyo, Japan).
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3

Immunohistochemical analysis of EBV

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Biopsies were fixed in 10% neutral buffered formalin, embedded in paraffin, and stained with hematoxylin–eosin. Immunohistochemical staining and in situ hybridization were performed on an automated stainer (Bond Max; Leica Biosystems). The presence of EBV was demonstrated by in situ hybridization with a probe for small RNA-encoding regions 1 and 2 (EBER) probe (Dako). Antibodies used were: anti-CD3 (polyclonal rabbit, CD3ε, A0452; Dako Agilent), anti-CD79a (monoclonal mouse IgG1k, JCB117; Dako), anti-caldesmon (monoclonal mouse IgG, clone h-CD [M3557]; Dako), anti-PAX5 (clone DAK-PAX5; 1/30; Dako), and anti-4-1BBL/CD137L (polyclonal rabbit IgG, ab64912; Abcam).
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4

In Situ Hybridization for EBV Detection

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To detect Epstein-Barr virus (EBV) infection, we stained 3 μm-thick sections for in situ hybridization according to the manufacturer's instructions. The EBER probe (#Y5200, Dako) was detected using a PNA ISH Detection Kit (#K5201, Dako). Only cases with a strong signal within more than 95% of tumor cell nuclei were interpreted as positive.
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