Log In Sign up
The largest database of trusted experimental protocols

Eber probe

Manufactured by Agilent Technologies
Visit Supplier
Sourced in United States

The EBER probe is a laboratory equipment used for the detection of Epstein-Barr virus (EBV) encoded RNA (EBER) in tissue samples. The EBER probe is a nucleic acid probe that binds to the EBER sequences present in EBV-infected cells, allowing for the identification of EBV infection through in situ hybridization techniques.

Automatically generated - may contain errors

Lab products found in correlation

Pna ish detection kit, by Agilent Technologies (2 mentions) Bond 3, by Leica (1 mentions) Ds u3 imaging system, by Nikon (1 mentions) Clone dak pax5, by Agilent Technologies (1 mentions) Bond max, by Leica (1 mentions) Anti cd3, by Agilent Technologies (1 mentions)

4 protocols using eber probe

1

EBER In-Situ Hybridization for EBV Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
To examine the EBV infection status, we stained 3‐μm‐thick sections for in situ hybridization. EBER probe (#Y5200; Dako) was detected using the PNA ISH Detection Kit (#K5201; Dako). Identifiable nuclear staining for EBER was interpreted as a positive result.
Tsuruta S., Kohashi K., Yamada Y., Fujiwara M., Koga Y., Ihara E., Ogawa Y., Oki E., Nakamura M, & Oda Y. (2020). Solid‐type poorly differentiated adenocarcinoma of the stomach: Deficiency of mismatch repair and SWI/SNF complex. Cancer Science, 111(3), 1008-1019.
+ Open protocol
+ Expand
2

EBV Detection in NKTCL Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
We detected the presence of EBV in NKTCL tissues by measuring EBV encoded small RNA (EBER) using ISH method. Slices were incubated with fluorescein-labeled EBER probe (Dako, Santa Clara, USA) and stained on an automated IHC and ISH systems Bond-III (Leica, Wetzlar, Germany) using Bond Polymer RedRefine Detection Kit (Leica, Wetzlar, Germany). Images were captured by Nikon DS-U3 imaging system (Nikon, Tokyo, Japan).
Sui X., Zhang C., Jiang Y., Zhou J., Xu C., Tang F., Chen B., Xu H., Wang S, & Wang X. (2020). Resveratrol activates DNA damage response through inhibition of polo-like kinase 1 (PLK1) in natural killer/T cell lymphoma. Annals of Translational Medicine, 8(11), 688.
+ Open protocol
+ Expand
3

Immunohistochemical analysis of EBV

Check if the same lab product or an alternative is used in the 5 most similar protocols
Biopsies were fixed in 10% neutral buffered formalin, embedded in paraffin, and stained with hematoxylin–eosin. Immunohistochemical staining and in situ hybridization were performed on an automated stainer (Bond Max; Leica Biosystems). The presence of EBV was demonstrated by in situ hybridization with a probe for small RNA-encoding regions 1 and 2 (EBER) probe (Dako). Antibodies used were: anti-CD3 (polyclonal rabbit, CD3ε, A0452; Dako Agilent), anti-CD79a (monoclonal mouse IgG1k, JCB117; Dako), anti-caldesmon (monoclonal mouse IgG, clone h-CD [M3557]; Dako), anti-PAX5 (clone DAK-PAX5; 1/30; Dako), and anti-4-1BBL/CD137L (polyclonal rabbit IgG, ab64912; Abcam).
Fournier B., Hoshino A., Bruneau J., Bachelet C., Fusaro M., Klifa R., Lévy R., Lenoir C., Soudais C., Picard C., Blanche S., Castelle M., Moshous D., Molina T., Defachelles A.S., Neven B, & Latour S. (2022). Inherited TNFSF9 deficiency causes broad Epstein–Barr virus infection with EBV+ smooth muscle tumors. The Journal of Experimental Medicine, 219(7), e20211682.
+ Open protocol
+ Expand
4

In Situ Hybridization for EBV Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
To detect Epstein-Barr virus (EBV) infection, we stained 3 μm-thick sections for in situ hybridization according to the manufacturer's instructions. The EBER probe (#Y5200, Dako) was detected using a PNA ISH Detection Kit (#K5201, Dako). Only cases with a strong signal within more than 95% of tumor cell nuclei were interpreted as positive.
Kawatoko S., Kohashi K., Torisu T., Sasaki T., Umekita S., Oki E., Nakamura M., Kitazono T, & Oda Y. (2022). Solid-type poorly differentiated adenocarcinoma of the stomach: A characteristic morphology reveals a distinctive immunoregulatory tumor microenvironment. Pathology, research and practice, 238.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!