Cell lines were as previously described [51 (link),52 (link)]. The DU145, 22Rv1 and LNCaP prostate cancer cell lines were cultured in RPMI-1640 medium (Gibco). PC3 cells were cultured in F12K (Kaighn’s) medium (Gibco, 21127022). All culture media were supplemented with 10% fetal bovine serum (FBS, Corning) and 1% antibiotics (penicillin/streptomycin, Gibco). As positive controls for induction of EMT genes or PD-L1, we treated the cells with transforming growth factor (TGF)-β or interferon (IFN)-γ (5 ng/mL), respectively, as we previously published [53 (link)]. Paraformaldehyde solution (AAJ19943K2, Thermo Scientific) and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; FluoroPure grade; D21490, Thermo Scientific) were used to fix and stain the nuclei. Recombinant Human Interferon gamma protein (Active) (ab259377), and Recombinant human TGF beta 1 protein (ab50036) were purchased from Abcam.
4 6 diamidino 2 phenylindole dihydrochloride dapi fluoropure grade
Manufactured by Thermo Fisher Scientific
4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; FluoroPure grade) is a fluorescent dye that binds strongly to DNA. It is commonly used in biological research applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab50036, by Abcam (2 mentions)
Paraformaldehyde solution aaj19943k2, by Thermo Fisher Scientific (2 mentions)
Recombinant human interferon gamma protein active ab259377, by Abcam (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Corning (2 mentions)
2 protocols using 4 6 diamidino 2 phenylindole dihydrochloride dapi fluoropure grade
1
Prostate Cancer Cell Culture Protocol
2
Prostate Cancer Cell Culture Protocol
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