Ng nitro l arginine methyl ester hydrochloride l name

Manufactured by Merck Group

Sourced in United States

NG-Nitro-l-arginine methyl ester hydrochloride (L-NAME) is a chemical compound used in various research applications. It serves as a nitric oxide synthase (NOS) inhibitor, which affects the production of nitric oxide in biological systems. The compound is utilized in scientific research, particularly in studies related to the cardiovascular system, neuroscience, and other areas of investigation.

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Close spelling variants of this product

Nitro-L-arginine methyl ester hydrochloride (L-NAME) NG-nitro-L-arginine Nitro-l-arginine L-NAME hydrochloride L-arginine hydrochloride L-NAME Arginine hydrochloride L-arginine Hydrochlorides

7 protocols using ng nitro l arginine methyl ester hydrochloride l name

Fluorescent Probe-Based Mitochondrial ROS Assay

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MitoSox red and DHE (Invitrogen, Paisley, UK) were diluted in DMSO prior to use. MEM, D-PBS, Apocynin, NG-Nitro-l-arginine methyl ester hydrochloride (L-NAME) and allopurinol were from the Sigma Chemical Co. MEM solution was made of (in mM) MgSO₄.H₂O (0.8), KCl (5.4), NaCl (116.4), NaH₂PO₄.H₂O (1), D-glucose (5.5), NaHCO₃ (26.2), HEPES (10), CaCl₂.2H₂O (1.9) and pH 7.4.

Pearson T., Kabayo T., Ng R., Chamberlain J., McArdle A, & Jackson M.J. (2014). Skeletal Muscle Contractions Induce Acute Changes in Cytosolic Superoxide, but Slower Responses in Mitochondrial Superoxide and Cellular Hydrogen Peroxide. PLoS ONE, 9(5), e96378.

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Volatile Organic Compound Exposure Assay

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1-octen-3-ol (99%), NG-Nitro-D-arginine-methyl ester hydrochloride (D-NAME), NG-Nitro-L-arginine-methyl ester hydrochloride (L-NAME), Minocycline (MC), toluene (≥99.9%), 1-butanol (99%) and Modified Griess reagent were purchased from Sigma, USA while NADPH and Nitroblue tetrazolium were supplied by Calbiochem and Alfa Aesar, USA respectively. The exposure protocol for 1-octen-3-ol was performed as described in Inamdar et al20 using 0.5 ppm (volume: volume) of undiluted liquid 1-octen-3-ol for all exposure experiments while toluene and 1-butanol were exposed at the concentration of 2.8 ppm (volume: volume) as described in Inamdar et al18 . The NO synthase inhibitors NG-nitro-L-arginine methyl ester hydrochloride (L-NAME), D-NAME and Minocycline were added at 2 mM concentration in agar food (2% agar and 5% sucrose).

Inamdar A.A, & Bennett J.W. (2014). A common fungal volatile organic compound induces a nitric oxide mediated inflammatory response in Drosophila melanogaster. Scientific Reports, 4, 3833.

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Kunming Mice in Electroacupuncture Study

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Forty healthy male Kunming mice weighing 20–22 g, average age of 3 months, were randomly allocated into EA, L-NAME, L-NAME EA, and control groups, 10 mice in each group. The animals were provided by the Animal Center of Chinese Academy of Medical Sciences. The animals were fed as experimental animals and treated according to the accepted international criteria. The experimental procedure should be conducted according to the Animal Ethics Committee of Chinese Academy of Medical Sciences.
The animals were anesthetized by intraperitoneal injection of 2% pentobarbital sodium (45 mg/kg⁻¹, Sigma-Aldrich, St. Louis, USA). Then the mice were injected intraperitoneally (i.p.) with physiological saline (15 mg kg⁻¹) in EA and control groups. The mice were injected intraperitoneally (i.p.) with 0.06% N^G-nitro-L-arginine methyl ester, hydrochloride (L-NAME) (15 mg kg⁻¹, Sigma Company, USA) in L-NAME and L-NAME EA groups. In addition, EA was given at bilateral Zusanli (ST36) after injection 40 min in EA and L-NAME EA groups' mice.

Wang S.Y., Zhang D., Tang L.M., Li S.Y., Wen M, & Song X.J. (2015). Effects of Electroacupuncture Stimulation at “Zusanli” Acupoint on Hepatic NO Release and Blood Perfusion in Mice. Evidence-based Complementary and Alternative Medicine : eCAM, 2015, 826805.

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Luteolin-induced Vascular Relaxation

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Luteolin was acquired from Supersmart USA, LLC (Miami, FL, USA). Acetylcholine (ACh), dimethyl sulfoxide (DMSO), indomethacin (non-selective COX inhibitor at 10 μM), N(G)-Nitro-L-arginine methyl ester hydrochloride (L-NAME, non-selective NOS inhibitor at 100 μM), phenylephrine (Phe), tetraethylammonium (TEA, a non-selective K⁺ channel blocker at 10 mM), and were obtained from Sigma (St. Louis, MO, USA). Potassium chloride (KCl) was purchased from Fisher Scientific (Waltham, MA, USA).
Luteolin was solubilized in 100 % DMSO to make a 2 mM stock concentration. This stock solution was added at increasing volumes of 2.5, 10, 12.5, 50, 50, and 100 μL to the 5 mL vessel baths containing physiological saline solution (PSS), which resulted in generation of the Luteolin-induced vasorelaxation curves. For the control vascular function curves, the same volume of DMSO was added to separate vessel segments isolated from the normal pregnant rats.

Yang W., Li Q., Duncan J.W., Bakrania B.A., Bradshaw J.L., Granger J.P., Rana S, & Spradley F.T. (2020). Luteolin-induced vasorelaxation in uterine arteries from normal pregnant rats. Pregnancy hypertension, 23, 11-17.

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Hesperidin-Based Vasodilation Study

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Hesperidin (purity ≥ 98%) was purchased from Chem Faces Company (Wuhan, Hubei, China). n(g)-Nitro-l-arginine methyl ester hydrochloride (l-NAME) and captopril were purchased from Sigma-Aldrich Corp (St. Louis, MO, USA). All the other chemicals used in this study were obtained from standard companies and were of analytical grade quality.

Maneesai P., Bunbupha S., Potue P., Berkban T., Kukongviriyapan U., Kukongviriyapan V., Prachaney P, & Pakdeechote P. (2018). Hesperidin Prevents Nitric Oxide Deficiency-Induced Cardiovascular Remodeling in Rats via Suppressing TGF-β1 and MMPs Protein Expression. Nutrients, 10(10), 1549.

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Oxygenated KRB Perfusion Protocol

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In mechanical and electrical recordings, experiments were performed in oxygenated KRB (in mmol/l): 120 NaCl; 5.9 KCl; 1.2 MgCl₂; 15.5 NaHCO₃; 1.2 NaH₂PO₄; 11.5 dextrose; and 2.5 CaCl₂ with 7.3–7.4 pH at 37.0 ± 0.5 °C. Thrombin was purchased from Alfa Aesar (Haverhill, MA, USA) and TTX, atropine and N^G-Nitro-l-arginine methyl ester hydrochloride (L-NAME) were purchased from Sigma (St Louis, MO, USA) and apamin was from Santa Cruz biotechnology, Inc (Dallas, TX, USA).

Sung T.S., Moon S.B., Perrino B.A., Sanders K.M, & Koh S.D. (2022). Altered functional responses by PAR1 agonist in murine dextran sodium sulphate-treated colon. Scientific Reports, 12, 16746.

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Pharmacological Modulation of Nociception

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Paracetamol, β-caryophyllene oxide, methiothepin, glibenclamide, [1 ,2 (link),4 (link)] oxadiazolo [4,3-a]quinoxalin-1-one (ODQ), N(G)-nitro-l-arginine methyl ester hydrochloride (l-NAME), and l-arginine were obtained from Sigma-Aldrich (St. Louis, MO, USA), and naloxone was acquired from Pisa Laboratories (Mexico City, Mexico). All drugs were suspended in carboxymethylcellulose (0.1%) and prepared minutes before being given to the animals. β-caryophyllene oxide and Paracetamol were administered orally (0.1 mL/10 g of body weight). Naloxone, methiothepin, l-NAME, ODQ, l-arginine, and glibenclamide were injected intraperitoneally (0.1 mL/10 g of body weight). Formalin (2%) was prepared by diluting aqueous formaldehyde to 37% from J.T. Baker (Matsonford Rd. Radnor Township, PA, USA).

Espinosa-Juárez J.V., Arrieta J., Briones-Aranda A., Cruz-Antonio L., López-Lorenzo Y, & Sánchez-Mendoza M.E. (2024). Synergistic Antinociceptive Effect of β-Caryophyllene Oxide in Combination with Paracetamol, and the Corresponding Gastroprotective Activity. Biomedicines, 12(5), 1037.

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