Nextseq 500 high output v2 kit 300 cycle kit

DNA was extracted from a peripheral blood sample using the phenol–chloroform method described by Green M.R. and Sambrook J. [18 ]. A Nanodrop 8000 spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, USA) was used to quantify the genetic material. Its integrity was evaluated with 2% agarose gel electrophoresis.
Libraries were prepared using the Nextera Rapid Capture Exome (Illumina^®, San Diego, CA, USA) and SureSelect Human All Exon V6 (Agilent Technologies, Santa Clara, CA, USA) kits, following the manufacturer’s recommendations. The sequencing reactions were performed on the NextSeq 500^® platform (Illumina^®, San Diego, CA, USA) using the NextSeq 500 High-output v2 Kit 300 cycle kit (Illumina^®, San Diego, CA, USA).

DNA was extracted from a peripheral blood sample using the phenol-chloroform method described by Sambrook et al. [28 ]. To quantify the genetic material, the Nanodrop-8000 spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, USA) was used and its integrity was evaluated by 2% agarose gel electrophoresis.
Libraries were prepared using the Nextera Rapid Capture Exome (Illumina^®, San Diego, CA, USA) and SureSelect Human All Exon V6 (Agilent technologies, Santa Clara, CA, USA) kits, following the manufacturer’s recommendations. The sequencing reactions were performed on the NextSeq 500^® platform (Illumina^®, San Diego, CA, USA) using the NextSeq 500 High-Output v2 Kit 300 cycle kit (Illumina^®, San Diego, CA, USA).

DNA extraction was performed according to the PhenolChloroform method [13 ] with modifications. The quantification of the extraction product was performed by Nanodrop-8000 spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, USA) and the prospective analysis of the quality of the extracted material was performed using 2% agarose gel electrophoresis.
The variant library (exome) was prepared using Nextera Rapid Capture Exome (Illumina^®, San Diego, CA, USA) and SureSelect Human All Exon V6 (Agilent Technologies, Santa Clara, CA, USA), following the kit protocol provided by the manufacturer. The sequencing reaction was performed by NextSeq 500^® platform (Illumina^®, San Diego, CA, USA) using the NextSeq 500 High-output v2 Kit 300 cycle kit (Illumina^®, San Diego, CA, USA).