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Competitive immunoassay

Manufactured by Cayman Chemical
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The Competitive Immunoassay is a laboratory equipment used for competitive binding analysis. It is designed to detect and quantify the presence of specific analytes in a sample.

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Competitive enzyme immunoassay kit (24 citations) Competitive enzyme immunoassay (6 citations) Competitive binding immunoassay (3 citations)

3 protocols using competitive immunoassay

1

Quantifying Platelet cGMP and cAMP Levels

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cGMP and cAMP content were determined in washed human platelets by a competitive immunoassay (Cayman Chemical, Hamburg, Germany) as per the manufacturer’s protocol.
Rukoyatkina N., Butt E., Subramanian H., Nikolaev V.O., Mindukshev I., Walter U., Gambaryan S, & Benz P.M. (2017). Protein kinase A activation by the anti-cancer drugs ABT-737 and thymoquinone is caspase-3-dependent and correlates with platelet inhibition and apoptosis. Cell Death & Disease, 8(6), e2898-.
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2

Urinary 8-isoprostane Measurement

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Lipid peroxidation was assessed in urine by competitive immunoassay (Cayman Chemical, Ann Arbor, MI, USA) measuring 8-isoprostane (8-iso-PGF2α) concentration following the manufacturer’s recommendations. The 8-iso-PGF2α concentrations were determined using a standard curve. Samples and standards were spectrophotometrically read at a wavelength of 412 nm.
Levenez M., Lambrechts K., Mrakic-Sposta S., Vezzoli A., Germonpré P., Pique H., Virgili F., Bosco G., Lafère P, & Balestra C. (2022). Full-Face Mask Use during SCUBA Diving Counters Related Oxidative Stress and Endothelial Dysfunction. International Journal of Environmental Research and Public Health, 19(2), 965.
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3

Urinary 8-Isoprostane Quantification

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Lipid peroxidation was assessed in urine by competitive immunoassay (Cayman Chemical, Ann Arbor, MI, USA) measuring 8-isoprostane (8-iso-PGF2α) concentration following the manufacturer’s recommendations. Briefly, 50 μL of urine was placed in a well plate with mouse monoclonal antibody.
Therefore, 50 μL of 8-iso PGF2α-tracer and 8-iso PGF2α-antiserum were added and incubated for 18 h at 4 °C. After washing with buffer, 200 μL of Ellman’s reagent containing the substrate of acetylcholinesterase was added.
The 8-iso-PGF2α concentrations were determined using a standard curve. Samples and standards were spectrophotometrically read at wavelengths between 405 and 420 nm.
Leveque C., Mrakic-Sposta S., Lafère P., Vezzoli A., Germonpré P., Beer A., Mievis S., Virgili F., Lambrechts K., Theunissen S., Guerrero F, & Balestra C. (2022). Oxidative Stress Response’s Kinetics after 60 Minutes at Different (30% or 100%) Normobaric Hyperoxia Exposures. International Journal of Molecular Sciences, 24(1), 664.
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