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P14arf

Manufactured by Cell Signaling Technology
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P14ARF is a laboratory protein that is used in cell biology research. It is a specific type of protein known as a cyclin-dependent kinase inhibitor, which plays a role in cell cycle regulation. P14ARF serves as a product for researchers to utilize in their experiments, but a detailed description of its intended use would require interpretation beyond the scope of this request.

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Lab products found in correlation

P16ink4a, by Cell Signaling Technology (2 mentions) P15ink4b, by Merck Group (1 mentions) Benchmark xt, by Roche (1 mentions) P16ink4a, by Roche (1 mentions) P16ink4a, by Santa Cruz Biotechnology (1 mentions) P21waf1, by Santa Cruz Biotechnology (1 mentions) Adriamycin, by Merck Group (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Etoposide, by Merck Group (1 mentions) Atorvastatin, by Cayman Chemical (1 mentions) Antibodies against p53, by Cell Signaling Technology (1 mentions) Trypan blue, by Merck Group (1 mentions) Recombinant human il 6, by Thermo Fisher Scientific (1 mentions) P stat3, by Cell Signaling Technology (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Propidium iodide, by Merck Group (1 mentions) P15ink4b, by Abcam (1 mentions) Total erk1 2, by Cell Signaling Technology (1 mentions)

4 protocols using p14arf

1

Immunohistochemical Analysis of Cell Cycle Regulators

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CRC tissue section review was performed independently by an experienced pathologist (J.-H. K.). A section of cut edge from fresh-frozen tissue was used for SA-β-Gal staining. The other section of cut edge from FFPE tissue was used for IHC analysis. IHC analysis was performed using a Benchmark XT automated processor (Ventana Medical Systems Inc, Tucson, AZ) on 4-μm-thick tissue sections of FFPE blocks. The following primary antibodies were used: p16INK4A, predilution (805–4713, Roche, Tucson, AZ); p15INK4B, 1:700 (SAB4500078, Sigma-Aldrich, Burlington, MA); p21Waf1, 1:300 (2990-1, Epitomics, Burlingame, CA); p27Kip1, 1:100 (NB110-66664, Novus Biologicals, Littleton, CO); p53, predilution (805–4713, Roche, Tucson, AZ); and p14ARF, 1:200 (74560, Cell Signaling Technology, Danvers, MA).
Park S.S., Lee Y.K., Park S.H., Lim S.B., Choi Y.W., Shin J.S., Kim Y.H., Kim J.H, & Park T.J. (2023). p15INK4B is an alternative marker of senescent tumor cells in colorectal cancer. Heliyon, 9(2), e13170.
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2

Western Blot Analysis of Cell Signaling

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Cells were treated with adriamycin (Sigma-Aldrich) or etoposide (Sigma-Aldrich), and SDS protein samples were then prepared by directly lysing the cells in SDS sample buffer (Elpisbiotech. Inc., Daejeon, Korea) after washing with ice-cold phosphate-buffered saline (PBS).
Western blots were conducted using antibodies against MDM2 (Santa Cruz Biotechnology, Inc., #sc-965), p21WAF1 (Santa Cruz Biotechnology, Inc., #sc-6246), p53 (Santa Cruz Biotechnology, Inc., #sc-126), p16INK4A (Santa Cruz Biotechnology, Inc., #sc-1661), p14ARF (Cell Signaling #74560), and GAPDH (Cell Signaling #2118).
Jeong Y.J., Cho J., Kwak J., Sung Y.H, & Kang B.C. (2022). Immortalization of primary marmoset skin fibroblasts by CRISPR-Cas9-mediated gene targeting. Animal Cells and Systems, 26(6), 266-274.
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3

Cellular Senescence Pathway Analysis

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TRIzol reagent was obtained from Invitrogen (Carlsbad, CA, USA). Atorvastatin was purchased from Cayman (Ann Arbor, MI, USA). Recombinant human IL-6 and a human IL-6 Standard ABTS enzyme-linked immunosorbent assay (ELISA) Development Kit were purchased from Peprotech (Rocky Hill, NJ, USA). Trypan blue and propidium iodide (PI) were obtained from Sigma (St. Louis, MO, USA). Antibodies against p53, p21, p27, p14ARF, p16INK4a, p-STAT3, STAT3, β-gal, and β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against TERT were purchased from EMD Millipore (Temecula, CA, USA).
Wang S.T., Huang S.W., Liu K.T., Lee T.Y., Shieh J.J, & Wu C.Y. (2020). Atorvastatin-induced senescence of hepatocellular carcinoma is mediated by downregulation of hTERT through the suppression of the IL-6/STAT3 pathway. Cell Death Discovery, 6, 17.
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4

Protein Expression Analysis Protocol

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Cell extracts were prepared by lysis in Laemmli buffer in the presence of protease inhibitor cocktail (Roche, Indianapolis, IN). The ZNF304 antibody was generated (by 21st Century Biochemicals, Marlboro, MA) against a peptide corresponding to amino acids GFWCEAEHEAPSEQSV. The following commercial antibodies were used: p14ARF (Cell Signaling Technology, Danvers, MA), p15INK4B (Abcam, Cambridge, MA), p16INK4A (Cell Signaling Technology), USP28 (Bethyl Laboratories, Montgomery, TX), PRKD1 (Cell Signaling Technology), phospho-ERK1/2 and total ERK1/2 (both from Cell Signaling Technology). The α-tubulin (TUBA) antibody was generated in-house.
Serra R.W., Fang M., Park S.M., Hutchinson L, & Green M.R. (2014). A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. eLife, 3, e02313.
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