Total protein (50 μg) was separated in a Bis-Trispolyacryl amide gel and transferred onto a PVDF membrane (Millipore). The membrane was then incubated in 5% bovine serum albumin (BSA), and then incubated with a primaryantibody at 4°C overnight. Next, samples were incubated with IRDye800CWHRP-conjugated anti-IgG at room temperature for 1hour and visualized via chemiluminescence with an infrared laser scanning system (OdysseyLicor,Lincoln,NE,USA). The following primary rabbit-anti-human antibodies were used: anti-ANGPT2 (1:1000; Abcam); anti-Notch1 (1:500; Abcam); anti-Notch2 (1:500; Abcam); anti-Runx2 (1:1000;Abcam); anti-Sp7/Osterix (1:2000; Abcam); anti-ALP (1:2000; Abcam); anti-OCN (1:500; Abcam) and anti-GAPDH (1:2,500; Abcam).
Anti angpt2
Manufactured by Abcam
Sourced in United States, United Kingdom
Anti-ANGPT2 is a primary antibody that specifically targets the Angiopoietin-2 (ANGPT2) protein. ANGPT2 is a growth factor involved in the regulation of angiogenesis and vascular remodeling. The Anti-ANGPT2 antibody can be used in various laboratory applications to detect and study the ANGPT2 protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti ocn, by Abcam (1 mentions)
Anti notch1, by Abcam (1 mentions)
Anti runx2, by Abcam (1 mentions)
Anti notch2, by Abcam (1 mentions)
Anti sp7 osterix, by Abcam (1 mentions)
Anti alp, by Abcam (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Anti gapdh, by Abcam (1 mentions)
Anti myc, by Proteintech (1 mentions)
Anti ha, by Proteintech (1 mentions)
Anti flag, by Merck Group (1 mentions)
Isopentane, by Merck Group (1 mentions)
Anti sox2, by Abcam (1 mentions)
Anti hunu, by Merck Group (1 mentions)
Tbs tween 20, by Merck Group (1 mentions)
Anti fgf 2, by Santa Cruz Biotechnology (1 mentions)
Anti pdgfrα, by Cell Signaling Technology (1 mentions)
Anti ng2, by Abcam (1 mentions)
Anti msi1, by Abcam (1 mentions)
Txrd conjugated goat anti mouse, by Southern Biotech (1 mentions)
4 protocols using anti angpt2
1
Western Blot Analysis of Cell Signaling Proteins
2
Protein Expression Analysis by Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total proteins were harvested from indicated cells and separated by SDS/PAGE gel and then transferred to PVDF membranes for 1 h. Subsequently, membranes were blocked by using 5% non-fat milk powder and then using the primary antibodies, anti-flag (Sigma, F3165), anti-MYBL1 (Affinity, AF9007), anti-ANGPT2 (Abcam, ab155106), anti-HA (Proteintech, 51064-2-AP), anti-myc (Proteintech, 16286-1-AP) and anti-PRMT5 (Proteintech, 18436-1-AP). Immunoreactive proteins were detected using a fluorescence imaging system (Minichemi 610, China).
3
Immunofluorescence Analysis of Mouse Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse brains were embedded in Tissue-Tek O.C.T. (Sakura Finetek, Alphen aan den Rijn, The Netherlands) and snap frozen in isopentane (Sigma-Aldrich) cooled on dry ice. Snap-frozen tissue was sectioned at 6 μm thickness on a cryostat (Leica CM3050S, Leica Microsystems, Wetzlar, Germany). Subsequent washes were done with TBS-Tween20 (Sigma-Aldrich) wash buffer, 3×3 min, all steps were performed at room temperature. The primary antibodies used were: anti-ANGPT2 (1:100, Abcam), anti-Msi1 (1:500, Abcam), anti-NG2 (1:100, Abcam), anti-Sox2 (1:100, Abcam), anti-VEGF (1:20, Abcam), anti-Vimentin (1:100, Abcam), anti-PDGFRα (1:100, Cell Signaling Technology, Beverly, MA, USA), anti-GFAP (1:500, Dako, Glostrup, Denmark), anti-Tubulin β3 (1:100, Millipore), anti-HuNu (1:100, Millipore), anti-FGF2 (1:100, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-IDH1 (Dianova, Hamburg, Germany) and anti-POU3F2 (SC-2895, Santa Cruz). The secondary antibodies used were: FITC-conjugated goat anti-rabbit (1:200, Southern Biotech), FITC-conjugated goat anti-mouse (1:200, Southern Biotech), TXRD-conjugated goat anti-mouse (1:100, Southern Biotech).
4
Western Blot Protein Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total protein was extracted from cells using lysis buffer, followed by determination of total protein concentration in the sample. Each protein sample (50 µl) was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and then transferred to polyvinylidene difluoride membranes. Membranes were blocked using 5% non-fat milk for 30 min at room temperature, then incubated with primary antibodies (1:1000 dilution; anti-ANGPT2, CD19, COL4A3, FGF18, ITGB4, ITGB8, LAMA3, LAMC2, PPP2R2C, SGK2, SYK, AKT3, COL6A1, CSF3, FGF1, ITGA2, ITGA11, MYB, PCK2, PGF, PIK3AP1, SGK1, TLR4 and TP53 (all Abcam, Cambridge, UK) at 4 °C overnight. Membranes were washed three times using 1 × Tris-buffered saline-Tween-20 (TBST), and then incubated with the corresponding horseradish peroxidase (HRP)-conjugated secondary antibody (1:5000 dilution; all Abcam) for 1 h at 37 °C, and again washed three times with 1 × TBST. GAPDH was quantified as the loading control. The blots were visualized using enhanced chemiluminescence (ECL) reagent (Cwbiotech, Beijing, China) and Image Lab software, version 5.1 (Bio-Rad Laboratories, Hercules, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!