Sterile millex filter unit

Manufactured by Merck Group

Sourced in Ireland, United States

The Sterile Millex Filter Unit is a laboratory filtration device designed to sterilize liquids and gases. It features a 0.22 μm membrane filter that removes bacteria and other microorganisms, ensuring a sterile output. The unit is available in various sizes and configurations to accommodate different flow rate requirements.

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Lab products found in correlation

Amicon ultra 15 centrifugal filter unit, by Merck Group (2 mentions) Crc 15r, by Mirion Technologies (1 mentions)

Close spelling variants of this product

Millex filter unit Millex filters Sterile filters Filter units

6 protocols using sterile millex filter unit

Avian Coronavirus Isolation in Eggs

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Tissue homogenate in PBS (1:10 dilution) was prepared from the organs with
Ultra-Turrax (IKA, Staufen, Germany), filtered through Sterile Millex Filter
Unit (22 μm, Merck Millipore, Carrigtwohill, Ireland), and 200 μl of the
homogenate was inoculated in the allantoic cavities of embryonated chicken eggs,
and incubated for 6–7 days at 37°C and candled daily. A sample was considered
positive for IBV if the embryos in the inoculated eggs showed typical lesions,
that is, stunting and curling of the embryos, and by PCR (Capua et al. 1999 ).

Kiss I., Mató T., Homonnay Z., Tatár-Kis T, & Palya V. (2016). Successive occurrence of recombinant infectious bronchitis virus strains in restricted area of Middle East. Virus Evolution, 2(2), vew021.

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Extraction of Recombinant Allergens from Transgenic Rice

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Transgenic‐rice seeds deposit the recombinant Cry j 1 and Cry j 2 in ER‐derived protein bodies in the endosperm. The protein bodies were isolated from TG‐rice seeds (Ozeki, Nishinomiya, Japan) and modified to make them powdery. Soluble allergens were extracted from powdered protein bodies as follows. First, the powdered protein bodies were dissolved in phosphate‐buffered saline (PBS) at a 1 : 150 ratio (w/v), and the mixture was sonicated on ice. Thereafter, the mixture was centrifuged at 5800 g for 10 min at 4°, and the supernatant was collected. The supernatant was then dialysed in PBS, concentrated 10‐fold using an Amicon Ultra‐15 Centrifugal Filter Unit (Merck Millipore, Co. Cork, UK), and sterilized through a 0·22‐μm Sterile Millex Filter Unit (Merck Millipore, Co.) to produce a filtered‐ and concentrated‐TG‐rice seed extract. Extraction from the protein body powder of non‐transgenic wild‐type rice (WT‐rice) seeds was performed in the same manner as extraction from the protein body powder of TG‐rice seeds.

Takaishi S., Saito S., Endo T., Asaka D., Wakasa Y., Takagi H., Ozawa K., Takaiwa F., Otori N, & Kojima H. (2019). T‐cell activation by transgenic rice seeds expressing the genetically modified Japanese cedar pollen allergens. Immunology, 158(2), 94-103.

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Radiolabeling of NM-scFv-DTPA for In Vivo Imaging

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The protocol of complexation between NM-scFv-DTPA and ¹¹¹InCl₃ was identical to that described in Section 2.5.1 with initial radioactivity of 138.26 ± 9.51 MBq. After the purification, as NMs were fluorescent-labeled, the elution profile of purified samples was established by measuring the NIRF intensity of each fraction. Besides, the radioactive elution profile of the purified samples was also established by measuring the radioactivity of each fraction with an activity meter (Capintec CRC-15R, Florham Park, NJ, USA). The six most concentrated fractions were mixed together, controlled in terms of pH, sterilized through a polyvinylidene fluoride membrane of 0.22 µm Sterile Millex^® Filter Unit (Merck Millipore, Carrigtwohill, Ireland) and used for IV injection into mice.
To determine the actual concentration of injected radiolabeled NM-scFv after the membrane sterilization, a calibration curve of NM-scFv-DTPA from 79.8 to 1.25 mg of iron/L according to its Dylight^TM680 NIRF intensity was established beforehand. The NIRF of the radiolabeled NMs used for IV injection was measured and the concentrations of the injected samples were calculated.

Nguyen P.V., Allard-Vannier E., Aubrey N., Labrugère-Sarroste C., Chourpa I., Sobilo J., Le Pape A, & Hervé-Aubert K. (2022). Radiolabeling, Quality Control and In Vivo Imaging of Multimodal Targeted Nanomedicines. Pharmaceutics, 14(12), 2679.

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Extracting Cry j 1 and Cry j 2 Allergens from Tg-Rice Seeds

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Tg-rice seeds deposit the deconstructed Cry j 1 and Cry j 2 in ER-derived protein bodies in the endosperm [15 (link)]. The protein bodies were isolated from Tg-rice seeds (Ozeki Corporation, Nishinomiya, Japan) and modified to make them powdery. Soluble allergens were extracted from powdered protein bodies as follows. First, the powdered protein bodies were dissolved in phosphate-buffered saline (PBS) at a 1:150 ratio (w/v), and the mixture was sonicated on ice. Thereafter, the mixture was centrifuged at 5800×g for 10 min at 4 °C, and the supernatant was collected. The supernatant was then dialyzed in PBS, concentrated tenfold using an Amicon Ultra-15 Centrifugal Filter Unit (Merck Millipore, Co. Cork, Ireland), and sterilized through a 0.22-µm Sterile Millex Filter Unit (Merck Millipore, Co. Cork, Ireland) to produce a filtered- and concentrated-Tg-rice seed extract. Extraction from the protein body powder of non-transgenic wild-type rice seeds (WT-rice seeds) was performed in the same manner as extraction from the protein body powder of Tg-rice seeds.

Takaishi S., Saito S., Kamada M., Otori N., Kojima H., Ozawa K, & Takaiwa F. (2019). Evaluation of basophil activation caused by transgenic rice seeds expressing whole T cell epitopes of the major Japanese cedar pollen allergens. Clinical and Translational Allergy, 9, 11.

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Isolation and Activation of Neutrophil-Derived Extracellular Vesicles

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Venous blood samples were drawn from healthy adult volunteers according to procedures approved by the National Ethical Committee (ETT-TUKEB No. BPR/021/01563-2/2015). Neutrophils were obtained by dextran sedimentation followed by a 62.5% (v/v) Ficoll gradient centrifugation (700g, 40 min, 22°C) as previously described (18 (link)). The preparations contained more than 95% PMN and <0.5% eosinophils. PMNs (typically 10⁷ cell in 1 mL HBSS) were incubated with or without activating agent for 30 min at 37°C in a linear shaker (80 rpm/min). After activation, cells were sedimented (500g, Hermle Z216MK 45° fixed angle rotor, 5 min, 4°C). Upper 500 μL of the supernatant was filtered through a 5 μm pore sterile filter (Sterile Millex Filter Unit, Millipore, Billerica, MA, USA). The filtered fraction was sedimented (15700 g, Hermle Z216MK 45° fixed angle rotor, 5 min, 4°C), and the pellet was carefully resuspended in the original incubation volume. Protein concentration of EV was determined by the Bradford protein assay using BSA as standard (5 (link)).

Lőrincz Á.M., Szeifert V., Bartos B., Szombath D., Mócsai A, & Ligeti E. (2019). Different Calcium and Src Family Kinase Signaling in Mac-1 Dependent Phagocytosis and Extracellular Vesicle Generation. Frontiers in Immunology, 10, 2942.

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Isolation of Antibacterial Neutrophil-derived EVs

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Venous blood was drawn from healthy adult volunteers according to procedures approved by the Institutional Review Board of the Semmelweis University. Neutrophils were obtained by dextran sedimentation followed by Ficoll-Paque gradient centrifugation as described previously (18 (link)). The preparation contained over 95% PMNs and less than 0.5% eosinophils.
For production of antibacterial EVs, PMNs (typically 4.5×10⁶ cell in 450 µL HBSS) were incubated with opsonized Zymozan A particles (5 mg added in 50 µL HBSS) for 30 minutes at 37°C on a linear shaker (80 rpm/min). After incubation, PMNs were sedimented (500×g, Hermle Z216MK 45° fixed angle rotor, 5 minutes, 4°C), and the supernatant was filtered through a 5 µm pore sterile filter (Sterile Millex Filter Unit, Millipore, Billerica, MA, USA). The filtered fraction was sedimented again (15,700×g, Hermle Z216MK 45° fixed angle rotor, 10 minutes, 4°C). The sediment was suspended in HBSS at the original incubation volume. Albumin concentration was 1 mg/mL (dissolved in HBSS) in the indicated samples.

Lőrincz Á.M., Timár C.I., Marosvári K.A., Veres D.S., Otrokocsi L., Kittel Á, & Ligeti E. (2014). Effect of storage on physical and functional properties of extracellular vesicles derived from neutrophilic granulocytes. Journal of Extracellular Vesicles, 3, 10.3402/jev.v3.25465.

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