Body fat content was measured using quantitative magnetic resonance (QMR) for flies at specific ages at the UAB Small Animal Phenotyping Core. Virgin flies were collected, sorted by sex and genotype, and aged for the desired time (3–5, 20, 30, 40 or 50 days old). At the appropriate age, flies were placed into a microcentrifuge tube (10 flies per tube, 4 tubes per sex and genotype for each time point), and stored on ice. QMR analysis was performed by the UAB Small Animal Phenotyping core with an EchoMRI 3-in-1 machine (Echo Medical Systems, Houston, TX). Flies were transferred into the biopsy tube and scanned using the biopsy setting with 9 primary accumulations. Data were analyzed in R using ANOVA with a Tukey’s HSD post hoc test [31 ].
Echomri 3 in 1 machine
Manufactured by Echo Medical Systems
The EchoMRI 3-in-1 machine is a versatile laboratory equipment designed for various medical and research applications. It is capable of performing three key functions: magnetic resonance imaging (MRI), ultrasound imaging, and echocardiography. The machine utilizes advanced technology to capture high-quality images and data for analysis and diagnosis.
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Lab products found in correlation
3 protocols using echomri 3 in 1 machine
1
Quantifying Fly Body Fat Content
2
Quantitative Magnetic Resonance Analysis of Fly Body Composition
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Body composition was analyzed by QMR at the UAB Small Animal Phenotyping Core as described previously95 (link). Briefly, virgin flies were collected and processed at 0–4 days of age. Ten flies were used per replicate, and four replicates per sex and genotype. Groups of flies were moved into the biopsy tube and scanned using the biopsy setting with nine primary accumulations with an EchoMRI 3-in-1 machine (Echo Medical Systems, Houston, TX).
3
Body Composition Analysis by Quantitative Magnetic Resonance in Flies
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GO term analysis was carried out using the PANTHER (Protein ANalysis THrough Evolutionary Relationships; version 15.0) Classification System (Mi, Muruganujan, Ebert, et al., 2019; Mi, Muruganujan, Huang, et al., 2019) . A false discovery rate (FDR; p<0.05) was used for multiple testing correction in the overrepresentation test (Fisher's Exact test).
Body composition analysis by quantitative magnetic resonance (QMR)
Body composition was analyzed by QMR at the UAB Small Animal Phenotyping Core as described previously (Mills et al., 2018) (link). Briefly, virgin flies were collected and processed at 0-4 days of age. Ten flies were used per replicate, and four replicates per sex and genotype.
Groups of flies were moved into the biopsy tube and scanned using the biopsy setting with nine primary accumulations with an EchoMRI 3-in-1 machine (Echo Medical Systems, Houston, TX).
Body composition analysis by quantitative magnetic resonance (QMR)
Body composition was analyzed by QMR at the UAB Small Animal Phenotyping Core as described previously (Mills et al., 2018) (link). Briefly, virgin flies were collected and processed at 0-4 days of age. Ten flies were used per replicate, and four replicates per sex and genotype.
Groups of flies were moved into the biopsy tube and scanned using the biopsy setting with nine primary accumulations with an EchoMRI 3-in-1 machine (Echo Medical Systems, Houston, TX).
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