Calu 3

The immortalized human ovarian surface epithelial cells T29 and their transformed counterparts T29Kt1 (with the introduction of KRAS^V12 into the immortalized cells) were reported previously28 (link) and were cultured in medium consisting of 1:1 MCDB105 medium and M199 medium (Sigma-Aldrich Co.) with 10% fetal bovine serum (HyClone) in the presence of 1% penicillin and streptomycin (HyClone). HCT-116 (p53^+/+) and HCT-116 (p53^−/−) were kindly provided by Dr Chuangui Wang (East China Normal University, Shanghai, China) and were maintained in DMEM with the same supplements. The culture medium of other cells used is listed in Supplementary Table 2. A549, H441, H358, H1299, H1975, PC9, H661, H522, HCT-15, LoVo, SW480, SW620, DLD-1, HT29, CaCo2, HCT-8, CCD-18Co, CCD841CoN, Panc-1, CFPAC-1 and BxPC-3 were obtained from the American Type Culture Collection (Manassas, VA, USA); Calu-1, Calu-3, WI-38, MRC-5, T84, LS174T, SW1116 and AsPC-1 were obtained from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). KRAS status identification by sequencing and limited genotyping in cancer cells was shown in Supplementary Table 2. Most of these cells were grown at 37 °C under a humidified 95:5 (%; v/v) mixture of air and CO₂. SW480 and SW620 cells were grown without CO₂. All of the cell lines were authenticated by short tandem repeat analysis.

The lung cancer cell lines (A549 and Calu3), BEAS-2B, and 293T cell lines were purchased from Shanghai Cell Bank, Chinese Academy of Sciences. A549 and Calu3 were cultured by Dulbecco's modified Eagle's medium (DMEM) (Sigma, USA) containing 10% FBS, 10 units/mL penicillin and 10 µg/mL streptomycin. Roswell Park Memorial Institute (RPMI) 1640 media (Sigma, USA) supplemented with 10% FBS, 10 units/mL penicillin, and 10 µg/mL streptomycin were utilized to culture BEAS-2B at 37 °C in a 5% CO₂ incubator. 293T cell line was cultured with DMEM high-glucose medium (Sigma, USA) including 10% FBS, 10 units/mL penicillin, and 10 µg/mL streptomycin.