PDCs were transferred to Oncotest as frozen vials. On site, the cells were thawed, the freezing medium was removed, and the cells were resuspended and transferred into T75 flasks. Cells were grown for 3 to 7 days in RPMI/10% FBS until the culture reached around 80% confluence. Cells were collected and counted, and 5 × 106 cells were injected into the hind flanks of NOD scid gamma (NOG) mice (Taconic). Tumors developed within 25 to 85 days after injection; these tumors were explanted, and viable portions of the tumors were cut into pieces and implanted subcutaneously into female NMRI nu/nu mice (Harlan Laboratories). This process was repeated in order to serially passage the respective models. From each passage, formalin-fixed, paraffin-embedded (FFPE) blocks were prepared, and tumor slices were stained with hematoxylin and eosin (H&E). Slides were scanned with a Hamamatsu slide scanner, and images were extracted using the Nanozoomer program from Hamamatsu. All animal handling and experiments with animals were in accordance with the guidelines set by the Samsung Biological Research Institute.
Nanozoomer program
Manufactured by Hamamatsu Photonics
The NanoZoomer is a digital slide scanning system that captures high-resolution images of microscope slides. It utilizes a high-speed and high-resolution camera to digitize the entire slide at multiple magnification levels, enabling detailed examination and analysis of the sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nanozoomer program
1
Establishment of Patient-Derived Xenograft Models
2
Xenograft Tumor Model Development and Characterization
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PDCs were transferred to OncoTest, Germany as frozen vials. On site, the cells were thawed, the freezing medium was removed, and the cells were resuspended and transferred into T75 flasks. Cells were grown for 3–7 days in RPMI/10% FBS until the culture reached around 80% confluence. Cells were collected and counted, and 5,106 cells were injected into the hind flanks of NOD scid gamma (NSG) mice (Jackson Laboratories). Tumors developed within 25–85 days after injection; these tumors were explanted, and viable portions of the tumors were cut into pieces and implanted subcutaneously into female NMRI nu/nu mice (Harlan Laboratories). This process was repeated in order to serially passage the respective models. From each passage, formalin-fixed, paraffin-embedded (FFPE) blocks were prepared, and tumor slices were stained with hematoxylin and eosin (H&E). Slides were scanned with a Hamamatsu slide scanner, and images were extracted using the Nanozoomer program from Hamamatsu. All animal handling and experiments with animals were in accordance with the guidelines set by the Samsung Biological Research Institute.
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