Rabbit monoclonal anti ha tag

Antibodies for Western blot analysis: rabbit monoclonal Anti-HA-tag (Cell Signaling Technology, at 1:1,000 dilution), Rabbit monoclonal anti-SYVN1 (Cell Signaling Technology, at 1:1,000 dilution), mouse monoclonal anti-GAPDH (Abcam, at 1:2,500 dilution) rabbit monoclonal Anti-HA (Cell Signaling, at 1:1,000 dilution), mouse monoclonal anti-RFP (Thermo Fisher Scientific, at 1:1,000 dilution), rabbit anti- Endoglin P3D1 (Santa Cruz Biotechnology, at 1:200 dilution), anti-Mouse IgG Peroxidase antibody (Sigma Aldrich, at 1:40,000 dilution), and anti-Rabbit IgG Peroxidase antibody (Sigma Aldrich, at 1:30,000 dilution).
Antibodies for immunofluorescence: mouse monoclonal anti-HA-tag (Cell signaling Technology, at 1: 200 dilution), rabbit polyclonal anti-calnexin (Santa Cruz Biotechnology, at 1 : 200 dilution), rabbit anti-Histone-H3 (Cell Signaling Technology, at 1:1,000 dilution), mouse monoclonal anti-HA (Cell Signaling Technology, at 1: 200 dilution), Alexa Fluor 568-goat anti-mouse IgG (Molecular Probes, at1:200 dilution), and Alexa Fluor 488-goat anti rabbit IgG (Molecular Probes, at 1:200dilution).

The following antibodies were used for western blot detection: Anti-MBP mouse monoclonal antibody (from NEB, #E8032S, 1:10,000 dilution), Anti-GST rabbit monoclonal antibody (from Cell Signalling Technology, #2625S, 1:1,000 dilution) anti-Rac1/2/3 rabbit antibody (Cell Signalling Technology, #2465). Antibodies used for Proximity Ligation Assay: mouse monoclonal anti-FLAG M2 (Sigma-Aldrich, #F3165, 1:800 dilution), rabbit monoclonal anti-HA tag (Cell Signalling Technology, #3724, 1:800 dilution). DAPI was used to stain for nucleic acid (Thermo Scientific, #62248, 1:1,000 dilution).