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Kb r7943 mesylate

Manufactured by Bio-Techne
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KB-R7943 mesylate is a chemical compound marketed by Bio-Techne for use in scientific research and laboratory settings. It is a small molecule that functions as a sodium-calcium exchanger (NCX) inhibitor, which can be used to study the role of NCX in various cellular processes. The product is supplied as a powder for reconstitution in appropriate solvents.

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Lab products found in correlation

Fura 2 am, by Thermo Fisher Scientific (3 mentions) Bapta am, by Bio-Techne (2 mentions) 2 apb, by Bio-Techne (2 mentions) Cariporide, by Merck Group (2 mentions) Amiloride, by Bio-Techne (2 mentions) Hydrochlorothiazide, by Merck Group (2 mentions) Calphostin c, by Merck Group (2 mentions) Furosemide, by Bio-Techne (2 mentions) Nickel chloride nicl2, by Merck Group (2 mentions) 1 2 bis o aminophenoxy ethane n n n n tetraacetic acid bapta am, by Bio-Techne (2 mentions) Benzamil hydrochloride hydrate, by Merck Group (2 mentions) Pluronic f 127, by Thermo Fisher Scientific (1 mentions) Mitomycin c, by Roche (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Goscript reverse transcription system, by Promega (1 mentions) Gotaq qpcr master mix, by Promega (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by AppliChem (1 mentions) Furosemide, by Merck Group (1 mentions) Swine anti rabbit hrp, by Agilent Technologies (1 mentions) Rabbit anti actin antibody, by Merck Group (1 mentions)

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KB-R7943 (16 citations)

9 protocols using kb r7943 mesylate

1

Characterization of Intracellular Calcium Signaling

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4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) was purchased from Fluka (Buchs, Switzerland). SN-6 and KB-R7943 mesylate were from Tocris Bioscience (Ellisville, MO, USA). Fura-2/AM was from Molecular Probes (Eugene, OR, USA). A solution of NCX1 inhibitor in DMSO (Sigma, St. Louis, MO, USA) and a solution of Fura-2/AM in pluronic F-127 (Molecular Probes, Eugene, OR, USA) were prepared. GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix were purchased from promega (Madison, WI, USA). Mitomycin C was from Roche (Nutley, NJ, USA). All other reagents were purchased from Sigma (St. Louis, MO, USA).
Wen J., Pang Y., Zhou T., Qi X., Zhao M., Xuan B., Meng X., Guo Y., Liu Q., Liang H., Li Y., Dong H, & Wang Y. (2016). Essential role of Na+/Ca2+ exchanger 1 in smoking-induced growth and migration of esophageal squamous cell carcinoma. Oncotarget, 7(39), 63816-63828.
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2

Stimulation and Inhibition of Ion Channels

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The stimulations were performed with LPS from Sigma-Aldrich (E. coli O111:B4; #2630) and NaCl from Merck (#1.06400.1000). The channel inhibitors were purchased from Tocris (KB-R7943 mesylate [#12144] [17 (link)], SEA0400 [#6164] [18 (link)], amiloride [#0890] [54 (link)], EIPA [#3378] [55 (link)], and GSK 2193874 [#5106] [56 (link)]), Biotrend (tetrodotoxin [#BN0518] [57 (link)] and furosemide [(#BG0201] [58 (link)]), Sigma-Aldrich (DAPI [#D8417-5MG] [59 (link)]), and AppliChem (NiCl2 [#A2199,1000] [19 (link)]). For western blotting, we used rabbit anti-NCX1 antibodies from Abcam (#ab151608), rabbit anti-NFAT5 antibodies from Thermo Fisher Scientific (#PA1-023), and rabbit anti-ACTIN antibodies from Sigma-Aldrich (#A2066). Swine anti-rabbit HRP (Dako; #p0399) was used as secondary antibody.
Neubert P., Homann A., Wendelborn D., Bär A.L., Krampert L., Trum M., Schröder A., Ebner S., Weichselbaum A., Schatz V., Linz P., Veelken R., Schulte-Schrepping J., Aschenbrenner A.C., Quast T., Kurts C., Geisberger S., Kunzelmann K., Hammer K., Binger K.J., Titze J., Müller D.N., Kolanus W., Schultze J.L., Wagner S, & Jantsch J. (2020). NCX1 represents an ionic Na+ sensing mechanism in macrophages. PLoS Biology, 18(6), e3000722.
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3

Ion Channel Modulators Protocol

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2-APB, KB-R7943 mesylate, BTP2 (YM 58483), and BAPTA-AM were purchased from Tocris Bioscience. Methoxyverapamil (D600), nitrotetrazolium blue (NBT), and 5-bromo-4-chloro-3-indolyl phosphate (BCIP) were purchased from Sigma-Aldrich. Synta66 and GSK7975A were purchased form Aobious. Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) solution (0.5 M, pH 8.0) was purchased from Invitrogen.
Chang-Graham A.L., Perry J.L., Strtak A.C., Ramachandran N.K., Criglar J.M., Philip A.A., Patton J.T., Estes M.K, & Hyser J.M. (2019). Rotavirus Calcium Dysregulation Manifests as Dynamic Calcium Signaling in the Cytoplasm and Endoplasmic Reticulum. Scientific Reports, 9, 10822.
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4

Pharmacological Regulation of Ion Transport

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Furosemide (20 µM Tocris Bioscience), hydrochlorothiazide (20 µM, Sigma), amiloride (20 µM, Tocris Bioscience), benzamil hydrochloride hydrate (10 µM, Sigma), KB-R7943 mesylate (2 nM, Tocris Bioscience), 1,2-bis(o-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid (BAPTA, AM) (Tocris Bioscience), calphostin C (20 nM, Sigma), cariporide (10 µM, Sigma), and nickel chloride (NiCl2) (10 mM, Sigma).
Barbaro N.R., Foss J.D., Kryshtal D.O., Tsyba N., Kumaresan S., Xiao L., Mernaugh R.L., Itani H.A., Loperena R., Chen W., Dikalov S., Titze J.M., Knollmann B.C., Harrison D.G, & Kirabo A. (2017). Dendritic Cell Amiloride-Sensitive Channels Mediate Sodium-Induced Inflammation and Hypertension. Cell reports, 21(4), 1009-1020.
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5

Antibody Sources and Reagents

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Antibodies were obtained from the following sources: anti-phospho-p53 (9284), and anti-γ-H2A.X (9718) IgGs were from Cell Signaling Technologies; anti-p21 (F-5), and anti-p53 (6243) IgGs were from Santa Cruz Biotechnology; anti-p16 ARC (mAb EP1551Y) was from Abcam. ATPγS, ARL 67156, KB-R7943 mesylate, U-73122, PPADS tetrasodium salt, 5-BDBD, A740003, NF-157, NF-546, BAPTA-AM, and CGS 15943 were from Tocris Bioscience. ATP, apyrase, caffeine, and quercetin were from Sigma-Aldrich. Silencer Select negative control siRNA and Silencer Select MCU siRNA were from Thermo Fisher Scientific. All other biochemical reagents were of the highest available purity and were commercially obtained.
Volonte D., Benson C.J., Daugherty S.L., Beckel J.M., Trebak M, & Galbiati F. (2024). Purinergic signaling promotes premature senescence. The Journal of Biological Chemistry, 300(4), 107145.
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6

Platelet Activation and Signaling Assay

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Hepes, apyrase, adrenaline [(−)-epinephrine (+)-bitartrate salt)], acetylsalicylic acid (ASA), bovine serum albumin (BSA), human fibrinogen, PGE1, LY 294002, wortmannin, digitonin, nigericin, EIPA, BCECF-AM, acetazolamide, indometacine, phentolamine, TRIS (tris(hydroxymethyl)aminomethane), DIDS, Ponceau Red, and TBST buffer—Millipore, Tirofiban (Aggrastat) were acquired from Sigma (Merck KGAA, Darmstadt, Germany). Type I collagen was acquired from Chrono-log. Phycoerythrin (PE)-antihuman CD41a antibody, FITC-antihuman CD62P (P-selectin), and FITC-annexin V were acquired from Becton Dickinson (Franklin Lakes, NJ, United States). Rauwolscine hydrochloride, PSB 0739, KB-R7943 mesylate, 2-APB, MRS 2500, SQ 22536, and BRL 44408 maleate were acquired from Tocris. BAPTA-AM was acquired from Abcam (Cambridge, UK). Fura-2-AM and AlexaFluor(AF)647-annexin V were acquired from Thermo Fisher Scientific (Waltham, MA, United States). Bovine thrombin was acquired from was acquired from Synthaverse S.A., Lublin, Poland. Other chemicals were acquired from Sigma (Merck).
Gołaszewska A., Misztal T., Kazberuk A, & Rusak T. (2024). Study on the Mechanism of the Adrenaline-Evoked Procoagulant Response in Human Platelets. International Journal of Molecular Sciences, 25(5), 2997.
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7

Pharmacological Regulation of Ion Transport

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Furosemide (20 µM Tocris Bioscience), hydrochlorothiazide (20 µM, Sigma), amiloride (20 µM, Tocris Bioscience), benzamil hydrochloride hydrate (10 µM, Sigma), KB-R7943 mesylate (2 nM, Tocris Bioscience), 1,2-bis(o-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid (BAPTA, AM) (Tocris Bioscience), calphostin C (20 nM, Sigma), cariporide (10 µM, Sigma), and nickel chloride (NiCl2) (10 mM, Sigma).
Barbaro N.R., Foss J.D., Kryshtal D.O., Tsyba N., Kumaresan S., Xiao L., Mernaugh R.L., Itani H.A., Loperena R., Chen W., Dikalov S., Titze J.M., Knollmann B.C., Harrison D.G, & Kirabo A. (2017). Dendritic Cell Amiloride-Sensitive Channels Mediate Sodium-Induced Inflammation and Hypertension. Cell reports, 21(4), 1009-1020.
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8

Neuronal Solution Perfusion Protocol

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Solutions were applied by gravity from a glass capillary (1.2 mm outer diameter) placed ~1 mm from the neuron under study. Solutions were switched manually using a low dead volume manifold upstream of the glass capillary. CNQX and Gabazine were supplied by Abcam. KB-R7943 Mesylate was supplied by Tocris. Creatine Phosphate was supplied by Santa Cruz Biotech. Cinacalcet was supplied by Toronto Research Chemicals and Tetrodotoxin by Alomone Other reagents were obtained from Sigma-Aldrich.
Martiszus B.J., Tsintsadze T., Chang W, & Smith S.M. (2021). Enhanced excitability of cortical neurons in low-divalent solutions is primarily mediated by altered voltage-dependence of voltage-gated sodium channels. eLife, 10, e67914.
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9

Intracellular Calcium Regulation in Wistar Rats

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Wistar rats were bred under standard conditions at the Centro de Biología Molecular Severo Ochoa (CBMSO) in accordance with procedures approved in the Directive 2010/63/EU of the European Union with approval of the Research Ethics Committee of the Universidad Autónoma de Madrid (Comité de Ética de la Investigación UAM, CEI-UAM). M2 acetylcholine receptor cDNA was a gift from Ana Ruíz Gómez (CBMSO, Spain). The intracellular calcium chelator BAPTA-AM (acid 1,2-Bis (2-aminophenoxy) ethane-N, N, N ′, N′-tetraacetic tetrakis (acetoxymethyl ester)) was from Calbiochem. Carbachol (carbamoylcholine), methacholine, atropine, EGTA (ethylene-bis (oxyethylene nitrile) tetraacetic acid tetrasodium), Dantrolene hydrochloride, 2-aminoethoxydiphenyl borate (2-APB), 4-aminopyridine were from Sigma-aldrich. Thapsigargin, CGP37157, KB-R7943 mesylate and tertiapin LQ were from Tocris Bioscience.
Jiménez E., Fornés A., Felipe R., Núñez E., Aragón C, & López-Corcuera B. (2022). Calcium-Dependent Regulation of the Neuronal Glycine Transporter GlyT2 by M2 Muscarinic Acetylcholine Receptors. Neurochemical research, 47(1).
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