Expression analysis technical manual
The Expression Analysis Technical Manual provides detailed information and guidelines for conducting expression analysis experiments. The manual covers topics such as sample preparation, data analysis, and interpretation of results, serving as a comprehensive reference for researchers and scientists working in the field of gene expression analysis.
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10 protocols using expression analysis technical manual
Quantitative Analysis of Apoptosis-Related Genes
Transcriptomic Profiling of Diabetic Nephropathy
Total RNA Extraction and Microarray Analysis
Affymetrix Microarray Gene Expression Analysis
AGO1 IP and Drosophila Transcriptome
Genome-wide Transcriptome Analysis by Microarray
RNA Extraction and Real-Time PCR Analysis
Example 11
RNA was extracted from cell pellets using RNeasy Mini Kit with on-column DNase digestion (Qiagen; Hilden, Germany). cDNA synthesis was performed as described in the Expression Analysis Technical Manual (Affymetrix; Santa Clara, Calif.). GAPDH was used as control gene and related gene expression (Bcl-2, Mcl-1, Bcl-xL, BID and BAX; all from IDT, Coralville, Iowa), was analyzed on an Applied Biosystems 7500 Real-Time PCR System using Taqman Universal PCR Master Mix #4304437 and Assay on Demand primer/probe kits (Applied Biosystems; Waltham, Mass.). For TLDA assay, TLDA v2.0 was performed on the 7900HT real-time PCR system (Applied Biosystems) according to the manufacturer's protocol. Average delta CT was acquired from the results for further analysis. PCR cycling conditions were performed as follows: 95° C. for 15 s and 60° C. for 1 min, 40 cycles and then 95° C. for 10 min. To normalize RNA input, Human RNU44 (Table 4) small RNA was used as an internal control.
Affymetrix Gene Expression Profiling Protocol
Genome-wide Transcriptome Analysis by Microarray
Microarray Analysis of Aging and Mutation
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