The lyophilized crude peptides were dissolved in distilled water and again filtered through a Millipore membrane filter (0.45 μm) and applied to a column (Φ25×1,200 mm; Amersham Pharmacia Biotech, Little Chalfont, UK) saturated in P-2 resin. The eluent was deionized with distilled water with a flow rate of 0.8 mL/min. The fractions were collected at 15 min intervals with a fraction collector (Amersham Pharmacia Biotech, Uppsala, Sweden) and all fractions were subjected to absorbance measurements at 220 nm to confirm the peptide concentration. The fractions were collected on the basis of molecular weight and subsequently lyophilized prior to the ACE inhibitory activity measurements.
Fraction collector
Manufactured by Cytiva
Sourced in Sweden
A fraction collector is a laboratory instrument used to automatically collect fractions of a liquid sample as it elutes from a chromatography system. It is designed to precisely divide the eluent into multiple collection vials or tubes, allowing the user to isolate and analyze specific components of the sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fraction collector
1
Peptide Purification and Characterization
2
Peptide Purification from A. ebracteatus Hydrolysate
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HPLC analysis with a UV detector (RP-HPLC) was performed to partially purified peptides from A. ebracteatus protein hydrolysate. The column employed was an ODS-2 HYPERSIL C18; 250×4.6 mm, 5 µm particle size (Thermo Fisher Scientific, Inc.). A gradient mobile phase of 0–50 min was applied, with HPLC-grade 0.1% trifluoroacetic acid (TFA) (Thermo Fisher Scientific, Inc.), acetonitrile (Thermo Fisher Scientific, Inc.) and water (90:10 v/v). The injected sample volume was 100 µl (17.05 µg protein/ml) and the flow rate was 1.00 ml/min. Detection was performed at 220 nm. Each fraction was collected using a fraction collector (Amersham Pharmacia Biotech). Finally, fractions were lyzed in 50 mM Tris/HCl buffer (Thermo Fisher Scientific, Inc.) and then lyophilized using a freeze dryer (LaboGene).
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