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Il 10

Manufactured by Enzo Life Sciences
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Sourced in United States

Interleukin-10 (IL-10) is a cytokine that plays a crucial role in regulating the immune system. It is produced by a variety of cell types, including T cells, B cells, and monocytes. IL-10 functions to inhibit the production of pro-inflammatory cytokines, thereby suppressing the inflammatory response.

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IL-10 (human) ELISA Kit (3 citations)

3 protocols using il 10

1

Supernatant Analysis of Ad-MSC and Macrophage Coculture

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Supernatants were collected from cocultures between Ad-MSCs and PBMC-derived macrophages. They were centrifuged at 4° for 10 min at 2000 g to remove MSU crystals. Supernatants were analyzed according to the manufacturer’s instructions for PGE2 (514010, Cayman Chemical, USA, MI) and IL-10 (ADI-901-036, ENZO, USA, NY).
Medina J.P., Bermejo-Álvarez I., Pérez-Baos S., Yáñez R., Fernández-García M., García-Olmo D., Mediero A., Herrero-Beaumont G, & Largo R. (2023). MSC therapy ameliorates experimental gouty arthritis hinting an early COX-2 induction. Frontiers in Immunology, 14, 1193179.
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2

Cytokine and Growth Factor ELISA

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ELISAs were performed according to the manufacturers’ protocol (R&D Systems, Minneapolis, MN: IL-1β, IL-6, IL-10, IL-33, PDGF, TGF-β, TNF-α; Enzo Life Sciences, Lausen, Switzerland: ET-1).
Bode D., Lindner D., Schwarzl M., Westermann D., Deissler P., Primessnig U., Hegemann N., Blatter L.A., van Linthout S., Tschöpe C., Schoenrath F., Soltani S., Stamm C., Duesterhoeft V., Rolim N., Wisløff U., Knosalla C., Falk V., Pieske B.M., Heinzel F.R, & Hohendanner F. (2019). The role of fibroblast – Cardiomyocyte interaction for atrial dysfunction in HFpEF and hypertensive heart disease. Journal of molecular and cellular cardiology, 131, 53-65.
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3

Cytokine Profiling in Macrophages

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ELISA was used to evaluate the IL-10, IL-12, IL-4, TNF-α, and INF-γ (Enzo Life Sciences, New York, NY, USA) contents in culture medium derived from macrophages. Following the manufacturer's instructions, each sample was evaluated in duplicate, and protein levels were normalized to the number of adherent cells.
Chen W., Tang D., Lin J., Huang X., Lin S., Shen G, & Dai Y. (2022). Exosomal circSHKBP1 participates in non-small cell lung cancer progression through PKM2-mediated glycolysis. Molecular Therapy Oncolytics, 24, 470-485.
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