14c phenytoin

Fetal Bovine Serum (FBS), Penicillin/streptomycin, glutamine, Dextran, Texas Red™ (3000, 10,000, 40,000 and 70,000 MW), B-27™ Supplement (50X), HEPES and Hoechst 33342 were from Invitrogen (Thermo Fisher, Monza, Italy). [¹⁴C]sucrose, [¹⁴C]mannitol, [³H]methyl-glucose, [³H]citalopram, [¹⁴C]leucine, [¹⁴C]phenytoin, [¹⁴C]phenylalanine, [¹⁴C]caffeine, [³H]daunomycin, [³H]testosterone, [³H] [D-Ala2]-Deltorphin II, [³H]propranolol, [³H]verapamil, [³H]prazosin and Microscint 20 Scintillation liquid were purchased from Perkin Elmer, Milan, Italy. [³H]vinblastine, [³H]L-DOPA, [³H]bupropion, [¹⁴C]taxol, [³H]gabapentin, [³H]indomethacin, [³H]raclopride, and [³H]flumazenil are from BIOTREND Chemikalien GmbH (Köln, Germany). Dimethyl sulfoxide (DMSO), lucifer yellow (LY), kynurenic acid, (GF120918), KO143, JPH203, phloretin, and triton X100 were purchased from Sigma-Aldrich, Milan, Italy. Aqueous solutions of 8% paraformaldehyde were obtained from Electron Microscopy Sciences (Hatfield, PA, USA).
Tissue-culture-treated multi-well plates and transwell filter inserts (1.12 cm² growth area, 0.4 µm pore size; transparent polyester) and Corning 96-well plates polystyrene were obtained from Corning (Sigma-Aldrich, Milan, Italy). Optiplate-96 and white Opaque 96-well microplate were from Perkin Elmer, Milan, Italy.

Fetal bovine serum, penicillin/streptomycin, glutamine, HEPES, fungizone, deoxyribonuclease I, and Hoechst 33342 were from Invitrogen (Thermo Fisher, Monza Italy). [14C]sucrose, [14C]mannitol, [3H]methyl-glucose, [14C]leucine, [14C]phenytoin, [14C]caffeine, [3H]digoxin, [3H]daunomycin, [3H]testosterone, [3H] [D-Ala2]-deltorphin II, [3H]propranolol, [3H]prazosin and Microscint 20 scintillation liquid were purchased from Perkin Elmer, Milan Italy and [3H]vinblastine, [14C]Taxol, [3H]methotrexate, [3H]gabapentin and [3H]flumazenil were from BIOTREND Chemikalien GmbH (Köln, Germany).
Fluorescein isothiocyanate-labelled FITC-dextran 40, dimethyl sulfoxide (DMSO), lucifer yellow, rhodamine-123, kynurenic acid, elacridar (GF120918), Y-27632 (ROCK inhibitor), and triton X100 were purchased from Sigma–Aldrich, Milan, Italy, and 8% paraformaldehyde aqueous solution was from Electron Microscopy Sciences (Hatfield, PA, USA).
CHDI compounds, denoted cp (A to J) and histone deacetylase (HDAC) inhibitor (cp K) were synthesized as previously described [10 (link),11 (link)].