Androgen receptor‐specific small interfering (si)RNA, siAR1 (HSS100619) and siAR2 (HSS179972) were synthesized by Thermo Fisher Scientific. A validated nontargeting siRNA (Stealth RNAi siRNA Negative Control Med GC Duplex #2) was also purchased from Thermo Fisher Scientific. For AR knockdown, LNCaP cells were plated into 6‐well plates at 1 × 105 cells/well before transfection with 10 n mol L−1 of siRNA for 24 hours. Total proteins were extracted at 0, 24, 48, 72 and 96 hours after transfection.
Hss100619
Manufactured by Thermo Fisher Scientific
Sourced in United States
The HSS100619 is a laboratory instrument designed for performing high-speed cell sorting. The core function of this equipment is to rapidly and accurately separate cells based on their specific characteristics, such as size, granularity, or fluorescent markers. The HSS100619 utilizes advanced flow cytometry technology to enable precise cell sorting, which can be beneficial for various applications in cell biology, immunology, and cell-based research.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hss100619
1
Androgen receptor knockdown in LNCaP cells
2
Silencing UNC5B and AR Genes
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UNC5B or AR siRNA was transfected using lipofectamine transfection reagent (Invitrogen, Tokyo, Japan), according to the manufacturer’s instructions. UNC5B siRNA (UNC5B si#1: 5′-UACGUGUUCACGGGCGAGUCCUAUU-3′, 5′-AAUAGGACUCGCCCGUGAACACGUA-3′, UNC5B si#2: 5′-UCCACAGAGCUCACCUGCAAGAUCU-3′, 5′-AGAUCUUGCAGGUGAGCUCUGUGGA-3′, UNC5B si#3: 5′-GAGGGCCAGAUAUUCCAGCUGCAUA-3′, 5′-UAUGCAGCUGGAAUAUCUGGCCCUC-3′) (HSS137260, 137261, 137262, stealth, Thermo Fisher Scientific, USA) and AR siRNA (AR si#1: 5′-GACUCCUUUGCAGCCUUGCUCUCUA-3′, 5′-UAGAGAGCAAGGCUGCAAAGGAGUC-3′, AR si#2: 5′-GAUGAAGCUUCUGGGUGUCACUAUG-3′, 5′-CAUAGUGACACCCAGAAGCUUCAUC-3′, AR si#3: 5′-CCGGAAGCUGAAGAAACUUGGUAAU-3′, 5′-AUUACCAAGUUUCUUCAGCUUCCGG-3′) (HSS100619, 179972, 179973, stealth, Thermo Fisher Scientific, USA). Non-targeting siRNA (Invitrogen) was used as control.
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