Polyclonal anti-Hcp2B serum was raised in New Zealand white rabbits through subcutaneous immunization, as described previously [14 (link)]. For Western blotting, bacterial samples were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (Amersham Pharmacia Biotech, Piscataway, NJ, USA) as described previously [14 (link), 47 (link)]. The proteins were reacted with the primary antibodies anti-Hcp2B or anti-DnaK (Enzo Life Sciences, Farmingdale, NY, USA), followed by horseradish peroxidase conjugated goat anti-mouse or goat anti-rabbit IgG secondary antibodies. The antigen–antibody complexes were visualized with chemiluminescence substrate (Amersham Pharmacia Biotech).
Chemiluminescence substrate
Manufactured by Cytiva
Sourced in United Kingdom
Chemiluminescence substrate is a reagent used in chemiluminescence detection techniques. It is designed to produce light emission when it reacts with specific target molecules, enabling the visualization and quantification of these targets.
Automatically generated - may contain errors
3 protocols using chemiluminescence substrate
1
Polyclonal Anti-Hcp2B Serum Production and Western Blot Analysis
2
Western Blot Analysis of Key Molecules
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SCLC cell lines were used for western blot (WB) analyses of the four key molecules. The primary antibodies used for WB analysis are listed in Table 1 . The membrane was washed and incubated with a secondary antibody conjugated with horseradish peroxidase for 1 h, and the immune complex was visualized with a chemiluminescence substrate (Amersham Pharmacia Biotech, Buckinghamshire, UK).
3
Western Blot Analysis of Cultured Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cultured cells were prepared for WB analysis, as described previously [27] . A list of the primary antibodies used is shown in Supplemental Table 1. The membrane was then washed and incubated with the respective secondary antibodies conjugated with horseradish peroxidase (Cell Signaling, Danvers, MA) for 1 hour, and the immunocomplex was visualized with chemiluminescence substrate (Amersham Pharmacia Biotech, Buckinghamshire, UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!