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αcd3 pb

Manufactured by BioLegend
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αCD3-PB is a fluorochrome-conjugated monoclonal antibody that binds to the CD3 antigen expressed on the surface of T cells. This product is intended for use in flow cytometry and other immunoassays.

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Lab products found in correlation

α cd11b pe cy7, by BioLegend (1 mentions) Anti γδtcr pe, by Thermo Fisher Scientific (1 mentions) αcd4 fitc, by BD (1 mentions) αcd3 bv421, by BioLegend (1 mentions) αcd11c apc, by BioLegend (1 mentions) Lsr 2, by BD (1 mentions) α tcrβ fitc, by BD (1 mentions) Live dead nir, by Thermo Fisher Scientific (1 mentions) αcd3 pecy7, by Thermo Fisher Scientific (1 mentions) αcd27 apc, by BD (1 mentions) αcd8 percpcy5, by BD (1 mentions) αcd45ra fitc, by BD (1 mentions) Facscanto 2, by BD (1 mentions) Aria 3, by BD (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions)

2 protocols using αcd3 pb

1

Murine T Cell Immunophenotyping Protocol

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Anti-γδTCR-PE, αTCRβ-APC-eFluor780, αCD11c-FITC, αCD45.1-BV421, αSlamf4-PE, αSlamf4-FITC and αGranzymeB-FITC (eBioscience, San Diego, CA); αCD4-FITC and αTCRβ-FITC (BD Biosciences, San Jose, CA). αCD3-PB, αCD3-BV421, αCD8α-APC, αCD8α-PE-Cy7, αCD8β-PE, αCD8β-PerCP/Cy5.5, αCD4-BV650, αSlamf2-PE, αCD45.2-AlexaFluor 647, αCD45.2 FITC, αCD11b-PECy7, αCD11c-APC, αI-A/I-E-PB, αCCR9-PE, and αα4β7-APC were purchased from Biolegend (San Diego, CA). Data were collected using a BD LSRII and analyzed with FlowJo software (Tree Star, Ashland, OR).
O’Keeffe M.S., Song J.H., Liao G., De Calisto J., Halibozek P.J., Mora J.R., Bhan A.K., Wang N., Reinecker H.C, & Terhorst C. (2015). SLAMF4 is a Negative Regulator of Expansion of Cytotoxic Intra-epithelial CD8+ T Cells That Maintains Homeostasis in the Small Intestine. Gastroenterology, 148(5), 991-1001.e4.
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2

Characterization of CD8+ T Cells

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CD8+ T cells were stained with tetramers for 1 h at room temperature in the dark. Cells were washed and surface stained for 30 min with αCD3-PeCy7 (1:50–1:100; eBiosciences #25-0038-42) or αCD3-PB (1:50–1:100; Biolegend #300431, SD, USA) with αCD8-PerCPCy5.5 (1:50; BD Biosciences #341051), αCD27-APC (1:50–1:100; BD Biosciences #337169), αCD45RA-FITC (1:50–1:100; BD Pharmingen #555488) and Live/Dead-NIR (1:1000; Molecular Probes #L10119) at 4 °C, then either fixed with 1% PFA (Electron Microscopy Sciences), and acquired on the BD FACS Canto II (BD Biosciences) or resuspended in sort buffer and single-cell sorted on the BD Aria III (BD Biosciences).
Grant E.J., Josephs T.M., Loh L., Clemens E.B., Sant S., Bharadwaj M., Chen W., Rossjohn J., Gras S, & Kedzierska K. (2018). Broad CD8+ T cell cross-recognition of distinct influenza A strains in humans. Nature Communications, 9, 5427.
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