Hydrogen tetrachloroaurate 3 hydrate haucl4

Recombinant human EGF was obtained from PeproTech (London, UK). Hydrogen tetrachloroaurate(III) hydrate (HAuCl₄) was purchased from Alfa Aesar (Heysham, Lancashire, UK). ¹¹¹InCl₃ was purchased from Mallinckrodt (the Netherlands). Cy3-NHS ester was obtained from GE Healthcare (Little Chalfont, Buckinghamshire, UK). Vectashield mounting medium with DAPI was purchased from Vector Laboratories (Peterborough, UK). Thermanox plastic coverslips (13 mm diameter) were purchased from Nalge Nunc International (Rochester, NY, USA). Matrigel was purchased from Corning (Tewksbury, MA, USA). MDA-MB-468 cells were purchased from ATCC. 231-H2N cells were a kind gift from Dr Robert Kerbel. All other chemicals were purchased from Sigma-Aldrich (Dorset, UK).

Tannic acid (TA, M_w = 1701.23 g·mol⁻¹, CAS 1401-55-4), ferroceneboronic acid ≥ 95% (FcB(OH)₂, M_w = 229.85 g·mol⁻¹, CAS 12152-94-2), glucose oxidase from Aspergillus Niger sp. Type X-S (GOx, ~168 U·mg⁻¹, M_w = 160 kDa, G7141, CAS 9001-37-0), horseradish peroxidase (HRP, ~250 U·mg⁻¹, M_w = 44 kDa, CAS 9003-99-0), hydrogen peroxide solution 30 wt.% (H₂O₂, M_w = 34.01 g·mol⁻¹, CAS 7722-84-1), Bradford reagent (reference B6916), and phosphate buffered saline tablets were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ferrocene methanol (FcOH ≥ 95%, Mw = 216.1 g·mol⁻¹, CAS 1273-86-5) was retrieved from FluoroChem (Hadfield, UK). Hydrogen tetrachloroaurate(III) hydrate (HAuCl₄, M_w = 393.83 g·mol⁻¹, CAS 27988-77-8), d-(+)-glucose anhydrous 99% (M_w = 180.16 g·mol⁻¹, CAS 50-99-7), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS 98 %, M_w = 548.68 g·mol⁻¹, CAS 30931-67-0) were purchased from Alfa Aesar (Haverhill, MA, USA). Sodium chloride ≥99.5% (NaCl, M_w = 58.44 g·mol⁻¹, CAS 7631-99-4), and potassium carbonate ≥99.5% (K₂CO₃, M_w) =138.21 g·mol⁻¹, CAS 209-529-3) were purchased from Fischer (Pittsburgh, PA). All chemicals were used as received and solution were prepared using Milli-Q water with 18.2 MΩ·cm resistivity.