Cryopreserved mononuclear cells from rhesus brain were thawed at room temperature, placed in fresh complete media (For splenic cells: RPMI supplemented with 10% HI-FBS, 1% L-glutamine, 1% penicillin-streptomycin; For brain tissue derived cells: DMEM supplemented with 10% HI-FBS, 1% L-glutamine, 1% penicillin-streptomycin) and treated with 2 units/mL of DNase I (Roche Diagnostics) for 15 minutes at 37°C. Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for non-human primates (Miltenyi Biotec CD45 Microbeads non-human primate) in accordance with the manufacturer’s protocol. Enriched CD45+ cells were stained for CD45 and a live dead marker for subsequent flow cytometric sorting. Live CD45+ cells were characterized and quantified on a BD FACSymphony cell analyzer and sorted utilizing a FACS Aria and suspended in RPMI for single cell RNA sequencing studies.
Cd45 microbeads non human primate
Manufactured by Miltenyi Biotec
CD45 Microbeads non-human primate are magnetic particles conjugated with antibodies specific to the CD45 antigen. They are used for the isolation and enrichment of CD45-positive cells from non-human primate samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cd45 microbeads non human primate
1
Isolation of Rhesus Brain-Derived CD45+ Cells
2
Enrichment of brain-derived CD45+ cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cryopreserved mononuclear cells from rhesus brain were thawed, placed in fresh complete media (For splenic cells: RPMI supplemented with 10% HI-FBS, 1% L-glutamine, 1% penicillin-streptomycin; For brain tissue derived cells: DMEM supplemented with 10% HI-FBS, 1% L-glutamine, 1% penicillin-streptomycin) and treated with 2 units/mL of DNase I (Roche Diagnostics) for 15 minutes at 37°C. Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for non-human primates (Miltenyi Biotec CD45 Microbeads non-human primate) in accordance with the manufacturer’s protocol. Enriched CD45+ cells were stained for CD45 and a live dead marker for subsequent flow cytometric sorting. Live CD45+ cells were characterized and quantified on a BD FACSymphony cell analyzer and sorted utilizing a FACS Aria and suspended in RPMI for single cell RNA sequencing studies.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!