Anti mouse cd40 fitc

Manufactured by BD

Sourced in United States

Anti-mouse CD40-FITC is a fluorescently labeled antibody that binds to the CD40 receptor on mouse cells. It is a tool used in flow cytometry and other immunological applications to detect and analyze CD40-expressing cells.

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Lab products found in correlation

Anti mouse cd86 percp, by BD (2 mentions) Anti mouse cd80 alexa fluor 647, by BD (2 mentions) Anti mouse 1 a 1 e mhc 2 pe, by BD (2 mentions) Anti mouse cd86 fitc, by BD (1 mentions) Facscalibur, by BD (1 mentions)

Close spelling variants of this product

FITC)-conjugated anti-mouse CD40, FITC-conjugated hamster anti-mouse CD40 (HM40–3, Anti-CD40-FITC Anti-mouse CD40 CD40-FITC Anti-CD40 Mouse anti

3 protocols using anti mouse cd40 fitc

Isolation and Characterization of Murine Dendritic Cells

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After immunomagnetic separation, the mean yield in three independent experiments was 5.8% of the total cells present in the spleen suspensions. The population of isolated cells averaged 97% of CD11c⁺ cells, of which 63% were MHCII⁺. The phenotypic profiles of DCs were evaluated by flow cytometry, by labeling with anti-mouse CD11c - APC (Clone: HL3), anti-mouse MHC-II-PE (130-091-368, Miltenyi Biotec), anti-mouseCD80-FITC (Clone: 16-10A1), anti-mouseCD86-FITC (Clone: GL1), and anti-mouseCD40-FITC (Clone: 3/23) according to the manufacturer's instructions (BD Bioscience, USA). The readings were performed using the FACSCalibur (Becton-Dickinson, Franklin Lakes, NJ, USA) flow cytometer located at the Institute of Biology of UNICAMP, using FCS Express 5 Plus, Research Edition software.

Paiatto L.N., Silva F.G., Yamada Á.T., Tamashiro W.M, & Simioni P.U. (2018). Adoptive transfer of dendritic cells expressing CD11c reduces the immunological response associated with experimental colitis in BALB/c mice. PLoS ONE, 13(5), e0196994.

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Immune Cell Activation Analysis

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RAW 264.7 macrophages and DC2.4 DCs were respectively seeded in 12-well plates and incubated overnight for experiment. Svg3 was transfected using Lipofectamine reagent at a final concentration of 25 nM. After treatment for 24 h, the cells were collected and stained with anti-mouse CD86-PerCp, anti-mouse CD80-Alexa Fluor 647, anti-mouse CD40-FITC, or anti-mouse I-A/I-E (MHC II)-PE, respectively, for flow cytometry (BD Canto).

Zhou S., Su T., Cheng F., Cole J., Liu X., Zhang B., Alam S., Liu J, & Zhu G. (2024). Engineering cGAS-agonistic oligonucleotides as therapeutics for cancer immunotherapy. Molecular Therapy. Nucleic Acids, 35(1), 102126.

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Macrophage and DC Activation Assay

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RAW 264.7 macrophages and DC2.4 DCs were respectively seeded in 12-well plate and incubated overnight for experiment. Svg3 was transfected using lipofectamine reagent at a final concentration of 25 nM. After treatment for 24 h, the cells were collected and stained with anti-mouse CD86-PerCp, anti-mouse CD80-Alexa Fluor 647, anti-mouse CD40-FITC, anti-mouse I-A/I-E (MHC II)-PE respectively for flow cytometry (BD Canto).

Zhou S., Su T., Cheng F., Cole J., Liu X., Zhang B., Alam S., Liu J, & Zhu G. (2023). Engineering cGAS-agonistic oligonucleotides as therapeutics and vaccine adjuvants for cancer immunotherapy. bioRxiv.

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