Materials include the following: Whatman filter paper grade #41 and grade #1 (GE Healthcare Life Sciences), gold (III) chloride hydrate (99.9%, Sigma-Aldrich), sodium citrate tribasic dihydrate (99%, Sigma-Aldrich), 30% poly(sodium 4-styrenesulfonate) solution (PSS, Sigma-Aldrich), bovine serum albumin (BSA ≥ 98%, VWR Chemicals), sucrose (99.5%, Sigma-Aldrich), Tween 20 (Sigma-Aldrich), 6-[Fluorescein-5(6)-carboxamido] hexanoic acid N-hydroxysuccinimide ester (NHS-FL 75%, Sigma-Aldrich), pyocyanin (98%, Sigma-Aldrich), high affinity mouse monoclonal IgG antibodies anti-pyocyanin (mAb122) produced in-house [16 (link)], PC1-BSA bioconjugate (produced in-house, hapten density = 10), goat anti-mouse-IgG/HRP (Dako Agilent), sodium bicarbonate (99.7%, Sigma-Aldrich), sodium carbonate (≥ 97%, VWR Chemicals), 30% hydrogen peroxide solution (Sigma-Aldrich), 3,3′,5,5′-tetramethylbenzidine (TMB, 99%, Thermo Fisher), sulfuric acid (95%, VWR Chemicals), Bradford reagent (VWR Chemicals), PD-10 Desalting Column (GE Healthcare), 96-well maxisorp ELISA microplates (Thermo Scientific). PBS refers to phosphate buffered saline (pH 7.4), PBST refers to PBS with 0.1% Tween 20, and PBS-BSA refers to PBS containing 5 mg·mL−1 BSA).
Pyocyanin
Manufactured by Agilent Technologies
pyocyanin is a laboratory reagent used to identify the presence of the bacterium Pseudomonas aeruginosa. It is a blue-green pigment produced by P. aeruginosa and can be used as a marker for the detection and identification of this microorganism in various samples.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti mouse igg hrp, by Agilent Technologies (2 mentions)
Sodium citrate tribasic dihydrate, by Merck Group (2 mentions)
Whatman filter paper grade 41 and grade 1, by GE Healthcare (2 mentions)
Poly sodium 4 styrenesulfonate solution pss, by Merck Group (2 mentions)
6 fluorescein 5 6 carboxamido hexanoic acid n hydroxysuccinimide, by Merck Group (2 mentions)
Nhs fl, by Merck Group (2 mentions)
Pc1 bsa bioconjugate, by Agilent Technologies (2 mentions)
96 well maxisorp elisa microplates, by Thermo Fisher Scientific (2 mentions)
Pd 10 desalting column, by GE Healthcare (2 mentions)
Sodium bicarbonate, by Merck Group (2 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Thermo Fisher Scientific (2 mentions)
Sucrose, by Merck Group (2 mentions)
Tween 20, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Avantor (2 mentions)
Hydrogen peroxide solution, by Merck Group (2 mentions)
Sulfuric acid, by Avantor (2 mentions)
Sodium carbonate, by Avantor (2 mentions)
Bradford reagent, by Avantor (2 mentions)
Gold 3 chloride hydrate, by Merck Group (2 mentions)
Pyocyanin, by Merck Group (2 mentions)
2 protocols using pyocyanin
1
Pyocyanin Detection Assay Development
2
Pyocyanin Detection Assay Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Materials include the following: Whatman filter paper grade #41 and grade #1 (GE Healthcare Life Sciences), gold (III) chloride hydrate (99.9%, Sigma-Aldrich), sodium citrate tribasic dihydrate (99%, Sigma-Aldrich), 30% poly(sodium 4-styrenesulfonate) solution (PSS, Sigma-Aldrich), bovine serum albumin (BSA ≥ 98%, VWR Chemicals), sucrose (99.5%, Sigma-Aldrich), Tween 20 (Sigma-Aldrich), 6-[Fluorescein-5(6)-carboxamido] hexanoic acid N-hydroxysuccinimide ester (NHS-FL 75%, Sigma-Aldrich), pyocyanin (98%, Sigma-Aldrich), high affinity mouse monoclonal IgG antibodies anti-pyocyanin (mAb122) produced in-house [16 (link)], PC1-BSA bioconjugate (produced in-house, hapten density = 10), goat anti-mouse-IgG/HRP (Dako Agilent), sodium bicarbonate (99.7%, Sigma-Aldrich), sodium carbonate (≥ 97%, VWR Chemicals), 30% hydrogen peroxide solution (Sigma-Aldrich), 3,3′,5,5′-tetramethylbenzidine (TMB, 99%, Thermo Fisher), sulfuric acid (95%, VWR Chemicals), Bradford reagent (VWR Chemicals), PD-10 Desalting Column (GE Healthcare), 96-well maxisorp ELISA microplates (Thermo Scientific). PBS refers to phosphate buffered saline (pH 7.4), PBST refers to PBS with 0.1% Tween 20, and PBS-BSA refers to PBS containing 5 mg·mL−1 BSA).
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