Peg bis amine

Manufactured by Merck Group

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PEG-bis(amine) is a chemical compound used in various laboratory applications. It is a polyethylene glycol (PEG) derivative with two terminal amine groups. PEG-bis(amine) is a versatile linker molecule that can be used to conjugate or modify other compounds in research and development.

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Amines (8 citations) PEG) amine (2 citations)

6 protocols using peg bis amine

Tunable Hydrogel Biomaterials for Cell Culture

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PEG bis(amine)
(average M_n 3400, Sigma-Aldrich, St. Louis,
MO), lyophilized RGD-ELP, and lyophilized RDG-ELP were solubilized
in chilled phosphate buffered saline (PBS) (1×, pH 7.4) at a
stock concentration of 12.5 wt % separately and dissolved by vortex.
Air bubbles were removed by centrifugation, and the final solutions
were kept on ice until use. An initial cross-linker stock solution
of 16.1 mg/mL tris(hydroxymethyl)phosphine (THP, Sigma-Aldrich) was
prepared in PBS and kept on ice. The stoichiometric cross-linking
ratio (X) of cross-linker reactive hydroxyl groups
(3 per THP molecule) to total primary amine groups (14 per ELP chain
and 2 per PEG chain) was varied by adjusting the concentration of
THP stock solution during the experiments. The hydrogel precursor
solutions were mixed with the cross-linker at a 4:1 volume ratio to
yield cross-linked hydrogels that consisted of 5 wt % ELP and 0, 1,
2.5, or 5 wt % of PEG. Cross-linking ratios of 1.10, 1.65, and 2.20
were used in different experiments. To control the density of RGD
ligands, RGD-ELP and RDG-ELP were mixed at different ratios while
maintaining a constant 5 wt % ELP.

Wang H., Cai L., Paul A., Enejder A, & Heilshorn S.C. (2014). Hybrid Elastin-like Polypeptide–Polyethylene Glycol (ELP-PEG) Hydrogels with Improved Transparency and Independent Control of Matrix Mechanics and Cell Ligand Density. Biomacromolecules, 15(9), 3421-3428.

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Synthesis and Characterization of Multifunctional Nanoparticles

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Folic Acid (FA), PEG-bis(amine) (Mn: 3.4 kDa), β-benzyl-L-aspartate (BLA), Triethylamine (TEA), Thiazolyl Blue Tetrazolium Bromide (MTT), Phosphate Buffered Saline (PBS), and Sodium Bicarbonate were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Triphosgene was purchased from Aldrich Chemical Co. (Milwaukee, WI, USA). N-hydroxysuccinimide (NHS) and N,N’-dicyclohexylcarbodiimide were purchased from Fluka (Buchs, Switzerland). Then, 3TT-IC-4Cl was provided by Zhongsheng Huateng Technology Co., Ltd. (Beijing, China) according to a previously reported method [47 (link)]. Indocyanine Green (ICG) was purchased from Adamas (Shanghai, China). CHCl₃ was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO) was purchased from Fuchen Chemical Reagent Co., Ltd. (Tianjin, China). Chloroform-d was purchased from Tenglong Weibo Technology Co., Ltd. (Qingdao, China). DMSO-d₆ was purchased from Ningbo Cuiying Chemical Technology Co., Ltd. (Ningbo, China). Dulbecco’s modified Eagle’s medium (DMEM), Fetal Bovine Serum (FBS), Penicillin and Streptomycin were purchased from Gibco BRL (Invitrogen Corp., Carlsbad, CA, USA). All other chemicals were of an analytical grade and used as received without further purification.

Zhao Y., He Z., Zhang Q., Wang J., Jia W., Jin L., Zhao L, & Lu Y. (2021). 880 nm NIR-Triggered Organic Small Molecular-Based Nanoparticles for Photothermal Therapy of Tumor. Nanomaterials, 11(3), 773.

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Multifunctional PLGA-PEG Nanoparticles

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Poly (lactide-co-glycolide) (PLGA) (RG 504 H, acid terminated, lactide:glycolide 50:50, Mw: 38,000), Poly(ethylene glycol) (PEG)-bis-amine (Mn: 10,000), Poly(vinyl alcohol) (Mw: 31,000–50,000), folate, fluorescein-5-isothiocyanate (FITC), N,N′-Dicyclohexylcarbodiimide (DCC), Dichloromethane (DCM) and sulfo-N-hydroxysuccinimide (NHS) were obtained from Sigma-Aldrich (St. Louise, MO, USA); disulfiram, methylene chloride, diethyl ether, methanol and dimethyl sulfoxide (DMSO) was obtained from Merck (Darmstadt, Germany); Fetal bovine serum (FBS), DMEM media, PBS buffer, Trypsin/EDTA and penicillin–streptomycin were purchased from GIBCO (Maryland, USA); MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assay kit was obtained from Roche (Mannhein, Germany).

Fasehee H., Dinarvand R., Ghavamzadeh A., Esfandyari-Manesh M., Moradian H., Faghihi S, & Ghaffari S.H. (2016). Delivery of disulfiram into breast cancer cells using folate-receptor-targeted PLGA-PEG nanoparticles: in vitro and in vivo investigations. Journal of Nanobiotechnology, 14, 32.

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Synthesis and Stabilization of PMAO-KI Nanoparticles

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Previously synthesized KI NPs in 3 mL of chloroform was added to the mixture and sonicated into suspension. After which 120 mg Poly(maleic anhydride alt-1-octadecene) (Sigma-Aldrich, 776866) in 3 mL chloroform (Sigma-Aldrich, C2432) and 1 mL PEG-bis-amine (43 mg in 10 mL chloroform) (Sigma-Aldrich, P9906) were added. The reaction was then mixed for 2 hrs. The solution was then poured into a round bottom flask. The solvent was removed under a rotary evaporator and gentle heat (<40 °C) until dry. 1.5 mL bis-hexamethyl triamine (43 mg in 10 mL chloroform) (Fisher Scientific, B181425G) was added to the flask and sonicated until the film was resuspended. The solution was then placed under Rotovap again until dry at room temperature. 50 mM Borate buffer is then added to cover film and sonicated until film is fully in solution. After which the solution is filtered to remove excessive aggregates then centrifuged at 10,000 rpm for 10 minutes. After which and the supernatant is discarded and the precipitate dried below 120 °C to avoid polymer softening. The dried and sealed PMAO-KI NPs can be stored for at least 6 months with no discernable loss in quality.

Cline B.L., Jiang W., Lee C., Cao Z., Yang X., Zhan S., Chong H., Zhang T., Han Z., Wu X., Yao L., Wang H., Zhang W., Li Z, & Xie J. (2021). Potassium Iodide Nanoparticles Enhance Radiotherapy against Breast Cancer by Exploiting the Sodium-Iodide Symporter. ACS nano, 15(11), 17401-17411.

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Photosensitizer-Conjugated Nanoparticles

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Yttrium(III) chloride hexahydrate (YCl₃·6H₂O), erbium(III) chloride hexahydrate (ErCl₃·6H2O), ytterbium(III) chloride hexahydrate (YbCl₃·6H₂O), ammonium fluoride (NH₄F), 4-(dimethylamino) pyridine (DMAP), and triphosgene oleic acid were purchased from Aldrich Chemical Co. (Milwaukee, WI, USA). Sodium borohydride, N,N’-dicyclohexylarbodiimide (DCC), and N-hydroxysuccinimide (NHS) were obtained from Fluka (Buchs, Switzerland). 4-Hydroxy-2-butanone was purchased from TCI (Tokyo, Japan). α-Lipoic acid (LA), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), folic acid (FA), β-benzyl-L-aspartate (BLA), triethylamine (TEA), hydrazine monohydrate, PEG-bis(amine) (molecular weight: 3.350 kDa), sodium bicarbonate were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Tetrahydrofuran (THF), n-hexane, benzene, N,N-dimethylformamide (DMF), methanol, chloroform, diethyl ether, dimethyl sulfoxide (DMSO), 1, 4-dioxane, acetic acid, and dichloromethane (DCM) were obtained from Samchun Pure Chemical Co., Ltd. (Gyeonggi-do, Korea). Pheophorbide a (Pha) was obtained from Frontier Scientific, Inc. (Logan, UT, USA).

Zhao L., Choi J., Lu Y, & Kim S.Y. (2020). NIR Photoregulated Theranostic System Based on Hexagonal-Phase Upconverting Nanoparticles for Tumor-Targeted Photodynamic Therapy and Fluorescence Imaging. Nanomaterials, 10(12), 2332.

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Synthesis of PEGylated Magnetic Nanoparticles

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The synthesized MPNs were redispersed in a vial with 1 mL of phosphate buffer saline (1x PBS, pH 7.4 unless otherwise specified). Subsequently, pyrocatechol (1.7 mg, 10 mmol) and hexamethylenediamine (HMDA, 2.6 mg, 10 mmol) were added under homogenous magnetic stirring (500 rpm) for the formation of the primer coating. The vials were then covered with a pierced Parafilm ® (Labbox, Madrid, Spain) in order to allow the entrance of oxygen to the reaction mixture. After 60 min, 2 mg of PEG bis(amine) (3000 MW, Sigma-Aldrich) was added to PEGylate the primer coating; during this step, the vial was sealed and kept under magnetic stirring (500 rpm) for 60 min. Finally, the MPN-PEGs were collected by centrifugation (5796 g, 1 min) and washed three times with water.

Suárez-García S., Esposito T.V.F., Neufeld-Peters J., Bergamo M., Yang H., Saatchi K., Schaffer P., Häfeli U.O., Ruiz-Molina D., Rodríguez-Rodríguez C, & Novio F. (2021). Hybrid Metal-Phenol Nanoparticles with Polydopamine-like Coating for PET/SPECT/CT Imaging. ACS applied materials & interfaces, 13(9).

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