Anti ccr2 pe

Manufactured by BioLegend

Anti-CCR2-PE is a fluorescently-labeled antibody that binds to the CCR2 chemokine receptor. CCR2 is a cell surface receptor that plays a role in the recruitment and migration of monocytes and macrophages. The PE (phycoerythrin) fluorescent label allows for the detection and analysis of CCR2-expressing cells using flow cytometry.

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Lab products found in correlation

Anti cd4 bv650, by BioLegend (1 mentions) Anti cxcr5 bv421, by BioLegend (1 mentions) Transcription factor fix perm buffer, by Thermo Fisher Scientific (1 mentions) Anti pd 1 pe dazzle 594, by BioLegend (1 mentions) Fixable viability dye efluor 455uv, by Thermo Fisher Scientific (1 mentions) Fortessa analyzer, by BD (1 mentions) Anti cd3 af700, by BioLegend (1 mentions) Accutase, by Merck Group (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Growth factor reduced matrigel, by BD (1 mentions)

3 protocols using anti ccr2 pe

CCR2 Expression Analysis via Flow Cytometry

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Cells were immunostained with CCR2 antibodies as previously described^{17 (link)}. Briefly, cells were incubated with anti-CCR2-PE (Biolegend, cat no. 357205) for 1 h, and analyzed using a LSRII Flow cytometer. Expression was normalized to unstained controls.

Fang W.B., Sofia Acevedo D., Smart C., Zinda B., Alissa N., Warren K., Fraga G., Huang L.C., Shyr Y., Li W., Xie L., Staggs V., Hong Y., Behbod F, & Cheng N. (2021). Expression of CCL2/CCR2 signaling proteins in breast carcinoma cells is associated with invasive progression. Scientific Reports, 11, 8708.

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Multiparametric Phenotyping of Immune Cells

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Synovial tissue and synovial fluid cells were thawed, washed twice in PBS, and incubated with Fixable Viability Dye eFluor 455UV (eBioscience) for 30 minutes. Cells were then washed in PBS/1%BSA and stained with antibodies against surface markers anti-CD3-AF700, anti-CD4-BV650, anti-CCR2-PE, anti-CXCR5-BV421, anti-PD-1-PE/Dazzle 594 (all Biolegend) for 30 minutes. Cells were washed once and incubated with eBioscience Transcription Factor Fix/Perm Buffer. Cells were washed in PBS/1%BSA/0.3% saponin and incubated in intracellular antibodies anti-MAF-PerCP-eFluor710 (sym0F1, eBioscience), anti-Bcl6-APC (BCL-UP, eBioscience), and anti-Blimp-1-AF488 (646702, R&D Systems) at 1:20 dilutions for 4 hours. Cells were washed once, filtered, and data acquired on a BD Fortessa analyzer. Intracellular detection of FoxP3 and CTLA-4 were performed by the same method on magnetic-bead purified blood CD4⁺ T cells using the indicated surface markers.

Rao D.A., Gurish M.F., Marshall J.L., Slowikowski K., Fonseka C.Y., Liu Y., Donlin L.T., Henderson L.A., Wei K., Mizoguchi F., Teslovich N.C., Weinblatt M.E., Massarotti E.M., Coblyn J.S., Helfgott S.M., Lee Y.C., Todd D.J., Bykerk V.P., Goodman S.M., Pernis A.B., Ivashkiv L.B., Karlson E.W., Nigrovic P.A., Filer A., Buckley C.D., Lederer J.A., Raychaudhuri S, & Brenner M.B. (2017). Pathologically expanded peripheral T helper cell subset drives B cells in rheumatoid arthritis. Nature, 542(7639), 110-114.

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Characterizing Breast Cancer Cell Spheroids

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500,000 cells were detached with Accutase (EMD Millipore, Cat no.SCR005). Cells were incubated for 30 min in PBS with anti-MET-FITC (0.1 mg/ml, SinoBiological, cat no.10692-R243-F) and/or anti-CCR2-PE (5ml/1million cells; Biolegend, cat no.357205). Cells were analyzed using a BD LSRII Flow Cytometer and normalized to respective unstained controls.
Spheroid growth assayCells were cultured in Collagen:Matrigel matrix using procedures previously described [26] . Briefly, rat tail collagen was mixed with 1:1 with Growth Factor Reduced Matrigel (BD Biosciences, cat no.354230) and coated on to 96-well plates. Plates were incubated with 2500 breast cancer cells/well in 200ml DMEM/10% FBS/2.5% Matrigel for 10 days. 4 fields/well at 10x magnification were captured using the FL-Auto EVOS imager. Sphere size was quantified using ImageJ and normalized to the total number of spheroids.

Acevedo D.S., Fang W.B., Rao V., Penmetcha V., Leyva H., Acosta G., Cote P., Brodine R., Swerdlow R., Tan L., Lorenzi P.L, & Cheng N. (2022). Regulation of growth, invasion and metabolism of breast ductal carcinoma through CCL2/CCR2 signaling interactions with MET receptor tyrosine kinases. Neoplasia (New York, N.Y.), 28, 100791.

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