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Human pcsk9 quantikine elisa kit

Manufactured by R&D Systems
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Sourced in United States

The Human PCSK9 Quantikine ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure human PCSK9 levels in cell culture supernates, serum, and plasma.

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Lab products found in correlation

Jca bm6070, by JEOL (1 mentions) Bio plex 200 system, by Bio-Rad (1 mentions) Infinity cholesterol reagent, by Thermo Fisher Scientific (1 mentions) Superose 6 10 300 gl column, by GE Healthcare (1 mentions) Cholesterol reagent set, by Horiba (1 mentions)

Close spelling variants of this product

PCSK9 Quantikine ELISA Kit PCSK9 ELISA kit Quantikine ELISA kit Quantikine ELISA Quantikine Human PCSK9 Quantikine kits Quantikines Human kits ELISAs

3 protocols using human pcsk9 quantikine elisa kit

1

Biomarker Profiling for Liver and Kidney Health

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Serum ALT, aspartate aminotransferase (AST), creatinine, urea nitrogen, total cholesterol, LDL-C, urinary GGT, and protein levels were measured with an automated analyzer (JCA-BM6070; JEOL, Tokyo, Japan). Serum PCSK9 protein levels were determined with the Human PCSK9 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA). Urinary Kim-1 levels were determined with xMAP technology using a Bio-Plex 200 Systems (Bio-Rad) kit according to the manufacturer’s instructions.
Wada F., Yamamoto T., Kobayashi T., Tachibana K., Ito K.R., Hamasaki M., Kayaba Y., Terada C., Yamayoshi A., Obika S, & Harada-Shiba M. (2021). Drug discovery and development scheme for liver-targeting bridged nucleic acid antisense oligonucleotides. Molecular Therapy. Nucleic Acids, 26, 957-969.
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2

PCSK9 Quantification in Serum

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Serum levels of PCSK9 were measured using the Human PCSK9 Quantikine ELISA Kit or Mouse PCSK9 Quantikine ELISA Kit (R&D Systems, Inc., Minneapolis, MN, USA), according to the manufacturer’s instructions. Human serum was diluted 1:20 and murine serum 1:200.
Ibold B., Tiemann J., Faust I., Ceglarek U., Dittrich J., Gorgels T.G., Bergen A.A., Vanakker O., Van Gils M., Knabbe C, & Hendig D. (2021). Genetic deletion of Abcc6 disturbs cholesterol homeostasis in mice. Scientific Reports, 11, 2137.
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3

Lipoprotein Cholesterol Analysis by Chromatography

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Lipoprotein cholesterol distributions were determined by on-line, high-performance gel-filtration chromatography with Infinity Cholesterol reagent (Thermo) at the Wake Forest University Lipid Core. Plasma total cholesterol and triglyceride were measured using the Cholesterol Reagent Set (Pointe Scientific) and Triglyceride Reagent and Free Glycerol Reagent (Sigma). Serum levels of PCSK9 were measured using the Human PCSK9 Quantikine ELISA kit from R&D Systems. For apolipoprotein analysis, an equal volume of plasma from each monkey was pooled and separated on a Superose 6 10/300 GL column (GE Healthcare). Fractions were resolved on SDS-PAGE gels, the proteins were transferred to a nitrocellulose membrane, and apolipoproteins were detected with goat anti-monkey affinity-purified antibodies.
Hong C., Marshall S.M., McDaniel A.L., Graham M., Layne J.D., Cai L., Scotti E., Boyadjian R., Kim J., Chamberlain B.T., Tangirala R.K., Jung M.E., Fong L., Lee R., Young S.G., Temel R.E, & Tontonoz P. (2014). The LXR–Idol axis differentially regulates plasma LDL levels in primates and mice. Cell metabolism, 20(5), 910-918.
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