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Amplex red cholesterol assay kit a12216

Manufactured by Thermo Fisher Scientific
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The Amplex Red Cholesterol Assay Kit A12216 is a fluorometric assay kit used to measure cholesterol levels. It provides a simple and sensitive method for detecting cholesterol in a variety of sample types, including biological samples. The kit utilizes the Amplex Red reagent, which reacts with hydrogen peroxide produced by the cholesterol oxidase enzyme to generate a fluorescent product that can be detected.

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Lab products found in correlation

Mak064, by Merck Group (1 mentions) Ab133116, by Abcam (1 mentions) Mak066, by Merck Group (1 mentions)

Spelling variants of this product

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2 protocols using amplex red cholesterol assay kit a12216

1

Measuring Metabolic Biomarkers in Cells

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Lactate (MAK064, Sigma-Aldrich), LDH (MAK066, Sigma-Aldrich), extracellular cholesterol (Amplex red cholesterol assay kit A12216, Invitrogen), intracellular cholesterol (cholesterol assay kit, cell-based, ab133116 Abcam), and ApoE (Apolipoprotein E human ELISA kit, EHAPOE, Invitrogen) concentrations or activity in media or in cells were measured following the manufacturer’s instructions.
Natarajaseenivasan K., Garcia A., Velusamy P., Shanmughapriya S, & Langford D. (2022). Citrate shuttling in astrocytes is required for processing cocaine-induced neuron-derived excess peroxidated fatty acids. iScience, 25(11), 105407.
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2

Quantifying Total Free Cholesterol

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The Amplex® Red Cholesterol Assay Kit A12216 (Invitrogen) was used to quantify total free cholesterol following the manufacturer’s instructions.
Schultz M.L., Fawaz M.V., Azaria R.D., Hollon T.C., Liu E.A., Kunkel T.J., Halseth T.A., Krus K.L., Ming R., Morin E.E., McLoughlin H.S., Bushart D.D., Paulson H.L., Shakkottai V.G., Orringer D.A., Schwendeman A.S, & Lieberman A.P. (2019). Synthetic high-density lipoprotein nanoparticles for the treatment of Niemann–Pick diseases. BMC Medicine, 17, 200.
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