All solvents and metabolite standards were obtained at highest purity. Hydrochloric acid [HCl, ≥32% (T), Fluka] and methanol (Chromasolv LC-MS Ultra) used for SPE were purchased from Honeywell (Seelze, Germany). For all other purposes, methanol [ultragradient high-performance liquid chromatography (HPLC)] was purchased from J. T. Baker (Norway). Acetonitrile (ULC/MS), methyl tert-butyl ether (MTBE, HPLC), hexane (HPLC), and formic acid (99%, ULC/MS) were purchased from Bio-Lab (Jerusalem, Israel). Acetone (≥99.8%, Chromasolv HPLC) was purchased from Sigma-Aldrich (Saint Louis, MO, USA). Water (HiPerSolv Chromanorm) used for SPE was purchased from VWR (Oslo, Norway). For all other purposes, water was purified by a Milli-Q system (resistivity of 18.2 megohm cm at 25°C, total organic carbon <5 parts per billion; Merck Millipore, Molsheim, France). Indole-3-acetic-2,2-d2 acid (d2-IAA; ≥98%) and N-hexanoyl-l -homoserine lactone-d3 (d3-C6-HSL; ≥99%) were used as isotopically labeled extraction standards (Santa Cruz Biotechnology, Dallas, TX, USA). Caffeine-(trimethyl-d9) (98%; Sigma-Aldrich) and l -tryptophan-d5 (98%; Cambridge Isotope Laboratories, Andover, MA, USA) were used as isotopically labeled injection standards for ultraperformance LC–HRMS (UPLC-HRMS) analysis.
Chromasolv hplc
Manufactured by Merck Group
Sourced in United States
Chromasolv HPLC is a high-performance liquid chromatography (HPLC) solvent and additive product line from Merck Group. It is designed for use in HPLC applications.
Automatically generated - may contain errors
2 protocols using chromasolv hplc
1
Metabolite Extraction and Analysis Protocol
2
Cyclic Voltammetry Characterization of Monomers
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For the cyclic voltammetry (CV) experiments, a three-electrode glass cell was used with a platinum wire as a working electrode, a platinum wire spiral as a counter electrode, and an Ag/Ag+ reference using 0.1 M Bu4NPF6 (TCI Europe, Zwijndrecht, Belgium) electrolyte solution in dichloromethane (DCM) (Chromasolv, HPLC; Sigma-Aldrich, St. Louis, MI, USA). The potential sweeps were controlled by Metrohm Autolab PGSTAT 100N potentiostat (Metrohm Autolab B.V., Utrecht, The Netherlands). The potential of the silver electrode was determined using a ferrocene redox couple (Fc/Fc+) for each measurement set, under the same conditions as the measured samples. The solutions were deaerated with argon before, and argon kept flowing into the cell, above the solution surface, during measurements. Two mmol/dm3 concentrations of the monomers were used for both the measurements and polymerization.
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