Tissue samples were fixed in 10% formalin and embedded in paraffin (FFPE), followed by staining of 3 μm-thick sections with hematoxylin and eosin. The immunohistochemistry for 12 EBVMCU cases was performed by an autoimmunostainer using BenchMark ULTRA (Ventana, Oro Valley, AZ, USA) and Leica Bond-III (Leica Biosystems, Wetzlar, Germany). Monoclonal antibodies were as follows; anti-CD3/LN10, CD20/L26, CD10/56C6, BCL6/LN22, EBNA2/PE2 (Novocastra Laboratories, Newcastle, UK), CD30/Ber-H2, MUM-1/MUM1p, LMP1/C.S1-4 (DAKO, Santa Fe, CA, USA), CD15/MMA (Becton Dickinson and Company, NJ, USA), PD-L1/E1J2J (Cell Signaling Technology, MA, USA), PD-L1/SP142 (Roche, Basel, CHE). EBV infection was evaluated by in situ hybridizations for EBV-encoded small RNA (EBER) oligonucleotide probe (PB0589, Leica Biosystems) on FFPE sections using Leica Bond-III.
Anti cd3 ln10
Manufactured by Leica Biosystems
Sourced in United Kingdom
The Anti-CD3/LN10 is a diagnostic tool used in immunohistochemistry (IHC) applications. It is designed to detect the presence of the CD3 antigen, which is a marker for T-lymphocytes. The product is intended for in vitro diagnostic use.
Automatically generated - may contain errors
Lab products found in correlation
Leica bond 3, by Leica Biosystems (1 mentions)
Cd30 ber h2, by Agilent Technologies (1 mentions)
Mum1 mum1p, by Agilent Technologies (1 mentions)
Cd20 l26, by Leica Biosystems (1 mentions)
Ebna2 pe2, by Leica Biosystems (1 mentions)
Cd10 56c6, by Leica Biosystems (1 mentions)
Bcl 6 ln22, by Leica Biosystems (1 mentions)
Lmp1 cs1 4, by Agilent Technologies (1 mentions)
Cd15 mma, by BD (1 mentions)
Pd l1 e1j2j, by Cell Signaling Technology (1 mentions)
Benchmark ultra, by Roche (1 mentions)
Anti pd l1 e1l3n, by Cell Signaling Technology (1 mentions)
Bond 3 stainer, by Leica Biosystems (1 mentions)
Nanozoomer xr, by Hamamatsu Photonics (1 mentions)
2 protocols using anti cd3 ln10
1
Immunohistochemical Analysis of EBV-Related Lymphomas
2
Immunohistochemical Analysis of PD-1, PD-L1, CD3, and CD8 in FFPE Tissue Sections
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Formalin-fixed paraffin-embedded (FFPE) tissue sections of 3 µm thickness were used for the immunohistochemical analyses. Staining for PD-1 and PD-L1 was conducted with anti-PD-1 (SP269, 1:50; Spring Bioscience) and anti-PD-L1 (E1L3N, 1:100; Cell Signaling Technology) antibodies, using a BOND-III stainer (Leica Biosystems). Staining for CD3 and CD8 was conducted with anti-CD3 (LN 10, 1:200; Novocastra) and anti-CD8 (SP16, 1:400; Thermo Scientific) antibodies using a Lab Vision Autostainer 480 (ImmunoVision Technologies Inc.). Signal visualization was done by diaminobenzidine, and sections were counterstained with haematoxylin. The slides were scanned with a NanoZoomer-XR (Hamamatsu Photonics) at 20× magnification (Figure 2 ).
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