Equal amounts of protein (30 μg) were separated by electrophoresis in precast 4-12% Bis-Tris Gels (Bio-Rad) and transferred to activated/pre-wetted PVDF membranes. The membranes were hybridized with the following primary antibodies as indicated: AT8 anti-P-tau pSer202/Thr205 (1:500, Thermo Scientific, #MN1020); anti-P-tau pS396 (1:1000, Abcam, #ab109390); anti-total tau T46 (1:1000, Thermo Scientific, #13-6400); anti-GAPDH (1:2000, Santa Cruz, #sc-32233); anti=P-CaMKII (1:1000, Abcam, #ab32678); anti-PSD-95 (1:1000, Millipore, #7E3-1B8), C1q (1:1000, Abcam, #ab182451). Secondary antibodies included: peroxidase labeled anti-mouse IgG (1:2000, Vector Laboratories); peroxidase labeled anti-rabbit IgG (1:2000, Vector Laboratories); peroxidase labeled anti-rat IgG (1/2000, Vector Laboratories). ECL (Pierce®) was used to reveal the immunoreactive proteins, and images were acquired using a Fujifilm ImageReader LAS-4000. Membranes were stripped using a stripping buffer (Thermo Scientific) when required. Luminescent immunoreactive protein bands were quantified using Fiji software (ImageJ).
Anti p tau ps396
Manufactured by Abcam
Anti-P-tau pS396 is a laboratory reagent used for the detection and analysis of phosphorylated tau protein at the serine 396 residue. This product is intended for research use only and its core function is to facilitate the study of tau protein phosphorylation, which is associated with various neurodegenerative disorders.
Automatically generated - may contain errors
Lab products found in correlation
Stripping buffer, by Thermo Fisher Scientific (2 mentions)
Image reader las 4000, by Fujifilm (2 mentions)
Anti gapdh, by Santa Cruz Biotechnology (2 mentions)
Bis tris gel, by Bio-Rad (2 mentions)
Anti psd95, by Merck Group (2 mentions)
Anti p camkii, by Abcam (2 mentions)
Peroxidase labeled anti rabbit igg, by Vector Laboratories (2 mentions)
Anti total tau t46, by Thermo Fisher Scientific (2 mentions)
Peroxidase labeled anti mouse igg, by Vector Laboratories (2 mentions)
Peroxidase labeled anti rat igg, by Vector Laboratories (2 mentions)
At8 anti p tau pser202 thr205, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti p tau ps396
1
Quantitative Protein Analysis in Tau Pathology
2
Western Blot Analysis of Tau and Synaptic Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Equal amounts of protein (30 μg) were separated by electrophoresis in precast 4–12% Bis-Tris Gels (Bio-Rad) and transferred to activated/pre-wetted polyvinylidene difluoride membranes. The membranes were hybridized with the following primary antibodies as indicated: AT8 anti-P-tau pSer202/Thr205 (1:500, Thermo Scientific, #MN1020); anti-P-tau pS396 (1:1000, Abcam, #ab109390); anti-total tau T46 (1:1000, Thermo Scientific, #13-6400); anti-GAPDH (1:2000, Santa Cruz, #sc-32233); anti-P-CaMKII (1:1000, Abcam, #ab32678); anti-PSD-95 (1:1000, Millipore, #7E3-1B8), C1q (1:1000, Abcam, #ab182451). Secondary antibodies included: peroxidase-labeled anti-mouse IgG (1:2000, Vector Laboratories); peroxidase-labeled anti-rabbit IgG (1:2000, Vector Laboratories); peroxidase-labeled anti-rat IgG (1:2000, Vector Laboratories). ECL (Pierce®) was used to reveal the immunoreactive proteins, and images were acquired using a Fujifilm ImageReader LAS-4000. Membranes were stripped using a stripping buffer (Thermo Scientific) when required. Luminescent immunoreactive protein bands were quantified using Fiji software (ImageJ).
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