Luminex analyzer

Manufactured by R&D Systems

Sourced in United States

The Luminex Analyzer is a multiplex detection system that utilizes flow cytometry technology to analyze multiple analytes simultaneously in a single sample. The core function of the Luminex Analyzer is to quantify the presence and concentration of specific proteins, nucleic acids, or other biomolecules in a sample.

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Lab products found in correlation

Xe 5000 hematology analyzer, by Sysmex (1 mentions)

2 protocols using luminex analyzer

Monocyte Counts and IL-4 Levels Measurement

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Monocyte counts were measured from venous blood samples using an XE‐5000 hematology analyzer (Sysmex, Vienna, Vienna, Austria). IL‐4 levels were measured previously in the Vienna CATS study,²⁴ using a Human IL‐4 Magnetic Luminex Assay (R&D Systems, Minneapolis, MN, USA) on a Luminex Analyzer.

Haider P., Kral‐Pointner J.B., Salzmann M., Moik F., Bleichert S., Schrottmaier W.C., Kaun C., Brekalo M., Fischer M.B., Speidl W.S., Hengstenberg C., Podesser B.K., Huber K., Pabinger I., Knapp S., Brombacher F., Brostjan C., Ay C., Wojta J, & Hohensinner P.J. (2022). Interleukin‐4 receptor alpha signaling regulates monocyte homeostasis. The FASEB Journal, 36(10), e22532.

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Luminex Assay for FSTL1 Protein Quantification

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Media were analyzed for FSTL1 protein levels using the Luminex magnetic bead-based multiplex assay (R&D Systems) according to the manufacturer’s instructions. In short, a standard curve was generated using calibrator diluent, and the standards and samples were transferred to a microplate. The microparticle cocktail was added to the wells, and the plate was incubated for 2 h at room temperature on a horizontal orbital microplate shaker, after which the plates were washed and consecutively incubated with the diluted Biotin-Antibody Cocktail and the diluted Streptavidin-PE cocktail. After resuspending the magnetic microparticles, the plates were read using a Luminex Analyzer (R&D Systems).

Peters M.C., Di Martino S., Boelens T., Qin J., van Mil A., Doevendans P.A., Chamuleau S.A., Sluijter J.P, & Neef K. (2022). Follistatin-like 1 promotes proliferation of matured human hypoxic iPSC-cardiomyocytes and is secreted by cardiac fibroblasts. Molecular Therapy. Methods & Clinical Development, 25, 3-16.

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