To carry out non-selective enrichment, 25 g of the sample was transferred into a
container with 225 mL BPW (Merck) and incubated at 37 ± 1°C for 16-20 h. Next 1 mL of the
sample was inoculated into 10 mL of Muller-Kauffmann tetrathionate-novobiocin broth
(MKTTn; Merck,) and incubated at 37 ± 1°C for 24 ± 3 h to carry out selective enrichment.
Subsequently, 0.1 mL of the same sample was placed into 10 mL of Rappaport Vasiliadis Soy
(RVS; Merck) broth and incubated at 41.5 ± 1°C for 24 ± 3 h. Incubated MKTTn and RVS broth
were streaked on xylose lysine deoxycholate agar (Merck) and Brilliance Salmonella agar
(Oxoid, England) and further incubated at 37 ± 1°C for 24 ± 3 h and 36 ± 1°C for 48 h,
respectively. The suspected Salmonella spp. was confirmed biochemically
using Microgen GN-ID A+B system in accordance with the manufacturer’s14 ) instruction (Microgen
Bioproducts, Camberley, UK).
container with 225 mL BPW (Merck) and incubated at 37 ± 1°C for 16-20 h. Next 1 mL of the
sample was inoculated into 10 mL of Muller-Kauffmann tetrathionate-novobiocin broth
(MKTTn; Merck,) and incubated at 37 ± 1°C for 24 ± 3 h to carry out selective enrichment.
Subsequently, 0.1 mL of the same sample was placed into 10 mL of Rappaport Vasiliadis Soy
(RVS; Merck) broth and incubated at 41.5 ± 1°C for 24 ± 3 h. Incubated MKTTn and RVS broth
were streaked on xylose lysine deoxycholate agar (Merck) and Brilliance Salmonella agar
(Oxoid, England) and further incubated at 37 ± 1°C for 24 ± 3 h and 36 ± 1°C for 48 h,
respectively. The suspected Salmonella spp. was confirmed biochemically
using Microgen GN-ID A+B system in accordance with the manufacturer’s14 ) instruction (Microgen
Bioproducts, Camberley, UK).