The sensitivity of carmoisine-based detection was verified by conducting ten-fold serial dilutions of RNA from SARS-CoV-2, SARS-CoV, and HCoV-OC43. RNA templates were incubated at 42 °C for 15 min, followed by another incubation at 65 °C for 45 min. The presence of RT-LAMP amplicons was verified by the addition of carmoisine. The results were validated using agarose gel electrophoresis. The gel was photographed under UV light using an imaging system, Bio-Rad Molecular Imager Gel ChemiDoc XR.
Molecular imager gel chemidoc xr
Manufactured by Bio-Rad
The Molecular Imager Gel ChemiDoc XR is a laboratory instrument designed for the detection and analysis of fluorescent and chemiluminescent signals in electrophoretic gels and blots. It features a high-resolution CCD camera and a specialized optical system to capture images of samples.
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Lab products found in correlation
2 protocols using molecular imager gel chemidoc xr
1
Sensitive SARS-CoV-2 Detection via RT-LAMP
2
Fluorescent Calcein Detection in LAMP
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Before starting the reaction, place the paper plate containing quenched calcein into the reaction chamber. After the reaction is complete, the pyrophosphate ions and manganese ions combine to show the fluorescence signal of calcein under UV radiation. LAMP amplicons were subjected to AGE for 30 min and then photographed under transparent UV radiation using the Bio-Rad Molecular Imager Gel Chemi Doc XR imaging system.
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