Rabbit monoclonal anti perk antibody

BSA (Bovine Serum Albumin) from Himedia (India), PBA and trypsin from Sigma, TUDCA and tunicamycin from Calbiochem (India) were used throughout this study except as otherwise mentioned. Rabbit polyclonal anti- BiP antibody (cat no# Sc-13698) and anti-eIF2α antibody (cat no# Sc-11386) were purchased from Santa Cruz Biotechnology, Inc, USA. Rabbit monoclonal phospho-eIF2α (ser-51) antibody (cat no# NB110-56949) was obtained from Novus Biologicals, USA. Rabbit monoclonal anti-PERK antibody (cat no# 5683S), rabbit monoclonal anti-ATF4 antibody (cat no# 11815S), mouse monoclonal anti-CHOP antibody (cat no# 2895S) and rabbit monoclonal anti-PARP antibody (cat no# 9532S) were bought from cell signaling technologies, USA. Mouse monoclonal anti-actin antibody (cat no# A5441-100UL) was obtained from Sigma.

Staining of pERK was performed as previously described^{18 (link)} using rabbit monoclonal anti-pERK antibody (4376; Cell Signaling Technology, Beverly, MA, USA) at a 100-fold dilution. Other staining experiments were also performed as previously described^{18 (link)}. The following primary antibodies were used: rabbit anti-CoRest antibody at a 100-fold dilution; rabbit monoclonal anti-myc antibody (2278; Cell Signaling Technology, Beverly, MA, USA) at a 100-fold dilution; mouse nc82 antibody (Developmental Studies Hybridoma Bank, Univ. Iowa, USA) for the detection of the presynaptic protein Bruchpilot at a 50-fold dilution; rabbit anti-DsRed antibody (632496; Takara, Shiga, Japan) at a 200-fold dilution; and chicken anti-GFP antibody (ab13970; Abcam, Cambridge, MA, USA) at a 2,000-fold dilution. The following secondary antibodies were used at 500-fold dilutions: donkey anti-chicken immunoglobulin Y (IgY) Alexa 488 antibody (703-545-155; Jackson ImmunoResearch Labs, Inc., West Grove, PA, USA), donkey anti-mouse IgG Alexa Fluor 488 antibody (715-545-150; Jackson ImmunoResearch Labs, Inc., West Grove, PA, USA), and donkey anti-rabbit IgG Cy3 antibody (711-166-152; Jackson ImmunoResearch Labs, Inc., West Grove, PA, USA). Images were captured using a confocal microscope LSM780 (Zeiss Microsystems, Jena, Germany) or FV1000 (Olympus, Tokyo, Japan).