Fibrils were pelleted by ultracentrifugation at 100,000 g for 4 hours (Beckman Coulter Optima MAX Ultracentrifuge, with TLA 120.2 rotor) and then the supernatant was removed. The hydrated pellet was packed into a glass capillary (0.7 mm) using a spatula and PBS buffer was inserted on both ends of the capillary by syringe. Capillaries were then sealed with capillary wax. A Rigaku FR-E generator (2kW, spot size 0.07 mm) with a source wavelength of 1.541780 Å was placed 65 mm from the sample and diffraction data was collected on a Rigaku Saturn 944 CCD camera (Tokyo, Japan) operating at −45 °C. The sample remained at room temperature during data collection. Diffraction datasets were analyzed in Structure Studio v. 2.2.3 r1 (Rigaku).
Saturn 944 ccd camera
Manufactured by Rigaku
Sourced in Japan
The Saturn 944 CCD camera is a high-performance X-ray detector designed for a variety of applications in crystallography and materials science. It features a large active area, high sensitivity, and low noise, making it suitable for a range of X-ray diffraction and scattering experiments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using saturn 944 ccd camera
1
X-ray Diffraction Analysis of Fibrils
2
Structural analysis of P23T hγD aggregates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aggregates of P23T hγD formed under acidic (pH 3) and neutral (pH 7) conditions were pelleted by centrifugation. Aggregates formed under neutral conditions were pelleted at 14,000 g for 20 min in a table-top VWR Galaxy 14D centrifuge, while aggregates formed under acidic conditions were pelleted by ultracentrifugation at 100,000 g for 4 h (Beckman Coulter Optima MAX Ultracentrifuge, with TLA 120.2 rotor). The majority of the supernatant was removed, and the pellet was resuspended in the remaining buffer and packed into a glass capillary (0.7 mm) using a syringe. Capillaries were sealed with wax to retain hydration of the samples. Diffraction data were measured at room temperature with a Rigaku Saturn 944 CCD camera (Tokyo, Japan). A Rigaku FR-E generator (2 kW, spot size 0.07 mm) was used as the X-ray source. Aggregates formed under neutral conditions were exposed for 75 s, while aggregates formed under acidic conditions were exposed for 210 s. Diffraction data were processed and analysed in Structure Studio (Rigaku).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!